VIIc3 Hanlin Pollinators

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Information about VIIc3 Hanlin Pollinators

Published on September 13, 2007

Author: AscotEdu


The Art of Pollination in Cages with Insects:  The Art of Pollination in Cages with Insects 'Everything there is to know about the bees and flies used in controlled pollination cages at NCRPIS, Ames, IA' S.J. Hanlin and S.G. McClurg Entomology Project November, 2005 Why do we use insect pollinators ? :  Why do we use insect pollinators ? The NCRPIS maintains collections of diverse germplasm. Controlled pollination of individual accessions helps retain the original genetic diversity of the plant populations. Some plants are more effectively pollinated by insects than by hand. In addition, insects may be more economical than hand pollination of some crops. At NCRPIS, insect pollinators are added to caged accessions of Brassica, miscellaneous umbels, sunflowers, and vegetables, as well as some ornamental and medicinal species. Primary insect pollinators used at NCRPIS:  Primary insect pollinators used at NCRPIS Honey bees or Apis mellifera Osmia lignaria or blue orchard bee and O. cornifrons or hornfaced bee Bumblebees or Bombus impatiens Houseflies or Musca domestica Blue bottle flies or Calliphora sp. Alfalfa leafcutter bee or Megachile rotundata Honey bees:  Honey bees Social bee with 2 - 4000 bees/cage Brought to U.S. by European colonists Traditionally used to pollinate many different plants Forage best from 15 to 32 C (60 to 90 F); don’t fly when wind speeds exceed 25 MPH Rearing well established but costly due to equipment and regular care required $82.50 for single story nuc $86.75 for double story nuc Aggressive, sting Honey bee management at NCRPIS:  Honey bee management at NCRPIS Source of bees: Maintain own colonies supplemented with purchased queens or packages in the spring as needed Work is done by controlled pollination supervisor and 2 to 3 crew members 6 outlying bee yards in the Ames, IA area 150 Parent colonies (3 story hives with 10 frames per story) 800 Nucleus hives (1-2 stories with 6 frames per story) Spring time Check health of overwintered bees Begin making specialized nucleus hives and queen cells in April Begin 'nuc' placement into field cages Summer time Continue making nucs and queen cells until mid August Place nucs into field cages at curators request (ca 90 weekly) Feed weekly with corn syrup solution and pollen patty bi-monthly Fall/Winter Monitor bees for mites; treat as needed Combine or discard queenless or very weak nucs Move strongest nucs and 2-story parent colonies to overwintering building Winterize 3-story parent colonies left outside Clean and repaint vacant equipment Honey bee use at NCRPIS:  Honey bee use at NCRPIS Major insect pollinator utilized Placed in ca 800 cages per year Can be used year round Used in both field and greenhouse cages Used to replace Osmia in Brassica cages Used in conjunction with flies in umbels What is a “nuc” ?:  What is a 'nuc' ? A 'nuc' or nucleus hive is a smaller version of a normal honey bee hive. Structure of nuc One or two stories with bees on frames of wax comb Bottom board with specially designed slide that allows bees to fly either direction from the box (inside or outside of cage) or to be confined within the box Lid with feeding hole Contents of single story = 6 frames total 2 frames with honey/pollen 3 frames with worker bees and brood (bee larvae) Queen bee 1 frame of empty comb Weighs ca 20 pounds Contains ca 2000 bees 2 story nuc Weighs ca 50 pounds Contains 4000 bees Frames contain: Lower box has brood and upper box has honey/empty comb Honeybee positioning into two cage sizes:  Honeybee positioning into two cage sizes Nucs are generally placed in the northwest corner or north end of the cage. Slide9:  Osmia:  Osmia Solitary bee; ca 40 bees per cage O. cornifrons brought from Japan in 1977, O. lignaria native to U.S. Excellent for early season plants (Brassica and fruit trees) Will work 10 to 32 C (50 to 90 F) during the spring Rearing is established; cannot be manipulated further Cost $17.50/small domicile Cannot relocate domiciles from original placement during pollination season Non-aggressive Osmia management at NCRPIS :  Osmia management at NCRPIS Source of bees: NCRPIS increase from 6 outlying locations and from small field cages Ca 1500 to 2500 bees are purchased annually from supplier in the U.S. Domicile is PVC pipe (5 or 7.6 cm diameter) suspended via eye-bolts from 1.25 m metal rod bent at 45 degree angle ca 1 m from bottom PVC end cap placed on back; front of pipe is cut at an angle to reduce weather damage to straws inside Small domiciles for germplasm cages contain 4 filled straws (ca 32 bee pupae) in bundle of 16 cardboard tubes Large domiciles for bee increase contain 5 filled straws (ca 40 bee pupae) in total of 23 cardboard tubes; front of domicile covered with mesh screen to protect from predators Bees need a source of mud for forming walls between cells in nesting straws Osmia domiciles are collected in early July and stored at 26 C (80 F) Domiciles must be handled carefully to prevent dislodging developing larvae from pollen balls within the nesting straws Nest straws will be invaded by a variety of Hymenoptera Mid-November straws are removed from domiciles and examined for bee pupae before winter storage at 4 C (40 F) In March straws are placed into new domiciles ready for springtime use Osmia use at NCRPIS:  Osmia use at NCRPIS Used in ca 200 field cages of Brassica annually from April – June Can be used in cool greenhouse cages beginning in April Used for some miscellaneous umbels and ornamentals Slide13:  Bumblebees:  Bumblebees Social bee with ca 50 bees/colony Use U.S. native species Excellent pollinator of many plants Work in rainy, cool (13 C or 55 F), windy weather Active for long hours Rearing is difficult so commercial colonies are used Expensive ($100 per unit) Mildly aggressive Bumblebee use at NCRPIS:  Bumblebee use at NCRPIS Bumblebees are used in ca 10 cages per year Used mainly for ornamentals with trumpet shaped flowers as bumblebees have long tongues Used in both field and greenhouse cages Can be purchased year round but availability may be limited by demand Queenright colonies may be too aggressive in working cages with tender or few flowers; Drone colonies are more 'mellow' Occasionally we collect 'wild' bumblebees for temporary cage use; put 3-5 bees per cage which live ca 5 days Slide16:  Houseflies and Blue Bottle flies:  Houseflies and Blue Bottle flies Place ca 200 flies per cage each week 'Incidental' pollinators Work at average temperatures Rearing is well established; pupae are low cost, cost varies per number of pupae purchased Require pre-conditioning of pupae prior to cage release for good emergence; no care required for adults Non-aggressive but may be considered 'irritating' Fly management at NCRPIS:  Fly management at NCRPIS Fly pupae are purchased BBF from Idaho every 2-3 weeks; stored at 0 C (32 F) for up to 3 weeks HF from ISU Ent project picked up weekly; cannot be stored Fly pupae are incubated on a weekly schedule 2-3 days at 26.5 C (80 F) Half-pint paper cartons with screened lids Better adult emergence Fly pupae HOLDERS One quart plastic containers weighted with plaster and exit hole cut out at top Gives flies a place to hide during inclement weather Protects pupae from people entering cages Use of flies at NCRPIS:  Use of flies at NCRPIS Flies are used primarily for pollination of Daucus and other umbels May use as 'fill in' pollinator for other crops (e.g. Erysimum, Crambe) when bees unavailable Used in ca 40 greenhouse cages in the winter and ca 20 GH cages in the spring/summer Used in ca 80 field cages in the summer Flies are replenished in cages weekly due to their short life span; live 2 to 3 weeks Using two species of flies together or flies along with HB has been shown to increase seed quantity and quality Slide20:  Alfalfa leafcutter bee:  Alfalfa leafcutter bee Why use ALC ? Pollinator to supplement honey bees Wanted insect easy to rear with established management Non-stinging preferred Largest unmet pollination demands: Wild cucumis in GH cages Late-blooming wild-type sunflowers Bee Biology/Systematics Lab, Logan, UT recommended ALC TRAITS of ALC: Solitary bee; use 20 to 40 bees per cage, replaced ca every other week Introduced to U.S. from Europe ca 1930 Traditionally used for pollination of forage legumes and blueberries Work at 26 C (80 F) or above but not frequently in cool cloudy or rainy weather, prefer dry sunny climate Rearing is established; bees are low cost ($100/gallon) and require little care Non-aggressive but will bite if squeezed Use of ALC bees at NCRPIS is still in the research stage !:  Use of ALC bees at NCRPIS is still in the research stage ! Lab work done 2004-2005 showed ALC can be used as greenhouse pollinator in winter/spring Early emergence of bees from cells is possible, but bees will not live for an extended time until a springtime 'trigger' occurs Continuous incubation allows for weekly replacement of bees Extended emergence test (into fall) is underway now We are collecting 'bare bees' rather than releasing them with cells as growers do Parasitoids (tiny wasps, ca 3 species) are a problem/concern We can release a known number of bees per cage ALC management: Cool (4 C or 40 F) storage and pre-incubation at room temperature:  ALC management: Cool (4 C or 40 F) storage and pre-incubation at room temperature Cells obtained from Canadian or western U.S. suppliers stored in screen trays or vented jars Cell depth should not exceed 3.8 cm (1.5 inches) for reduced mortality Pre-incubation at 23-24 C (73-75 F) for several days prior to warm incubation ALC management: warm incubation and bee collection:  ALC management: warm incubation and bee collection Cells incubated for 30 days at 30 C (86 F) for complete bee emergence First two weeks incubated in dark, then placed in chamber with limited light period Bees collected daily; provided wood nest cell and sucrose soaked wicks Retrieved ca 43 sets of bees (20 bees/set) from ca 2400 cells Parasitoids are controlled with 24 hour black-light/water trap Domiciles seem important in extending life span and activity level of ALC bees:  Domiciles seem important in extending life span and activity level of ALC bees Female bees carry pollen; providing domicile encourages them to work flowers in order to nest Cut disks from leaves and petals for nest cells Manmade domiciles have evolved from wood to styrofoam blocks; some models we tried in 2005 field and GH 2004 – 2005 Field Tests:  2004 – 2005 Field Tests 2004: Compared HB to ALC in cages 8 accessions No significant difference in quantity or quality of seed produced 2005: Compared HB to ALC with and without alfalfa nesting plant 6 accessions Seed processing is in progress Accessions ALC appeared highly attracted to in 2005 field/GH cages:  Accessions ALC appeared highly attracted to in 2005 field/GH cages ALC seem to be most effective at pollinating small to medium sized flowers of a 'flat' nature Angelica Brassica napus Cucumis Daucus Helianthus (wild-type heads) Melilotus Ocimum Potentilla Cucumis photo courtesy of L. Clark, NCRPIS Daucus umbel pollinated by ALC International Pollination Symposium 2006:  International Pollination Symposium 2006 July 23 – 27, Iowa State University, Ames, IA 'Host-Pollinator Biology Relationships - Diversity in Action' Questions ? :  Questions ?

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