Published on September 17, 2009
Elute AflaToxin with 1.0 ml Methanol Add Developer Solution Dilute with H2O Measure in Fluorometer Inject into HPLC
Vicam Immunoaffinity Column vs. ELISA
Training Vicam IA: Can be trained in an hour. Concept of method easy to understand. ELISA: Operator must measure and mix very small volumes, which can bead to errors. Concept of method difficult to understand.
Time to run test Vicam IA: Less than 15 minutes. ELISA: 20 minutes or more. Test requires 2 incubations of 5 or 10 minutes.
Storage of products Vicam IA: Room temperature and shelf life up to 1.5 years from date of production. ELISA: Must be refrigerated and 6 month shelf life. Must throwing away expired product.
Standards Vicam IA: Reusable standards to calibrate the fluorometer. One time low cost. ELISA: Must run 3 to 5 standards with every batch. Customer must pay to run each standard, which is costly.
Data display Vicam IA: Fluorometer printout in ppb or ppm for each sample. ELISA: Data printout in groups or must be calculated by using a separate computer and special software.
Ordering Vicam IA: Can order supplies separately as needed. ELISA: Must order as a kit. ELISA Wells can only be used with reagents in the kit (matched reagents).
Additional Advantage Vicam IA: • LOD: 0.5ppb. • Can be used with an HPLC clean up. ELISA: • LOD: 1 - 5 ppb • Can’t be used for HPLC clean up
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