Using protease to enhance the utilization of corn amino acids by broilers

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Information about Using protease to enhance the utilization of corn amino acids by broilers
Health & Medicine

Published on January 30, 2014

Author: DSM_Animal_Nutrition

Source: slideshare.net

Description

Research on the effect of protease (RONOZYME ProAct) in improving Amino Acid digestibility of corn, which is widely utilized in broiler diets. This would result in more energy utilization. Read the presentation to find out the research results.

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Use of a protease to enhance the utilization of corn amino acids by broilers. J.C.C. Carvalho1; R.L. Rios1; F.R. Mesquita1; E.M.C. Lima1; A.G. Bertechini1; J.O.B. Sorbara*2 Universidade Federal de Lavras. MG. Brazil 2 DSM Nutritional Products. São Paulo. SP. Brazil 1

Abstract

Introduction • Corn – mostly utilized cereal in broiler diets • Main energy source in the feed • Also contributes with 10 to 20% of total CP and AAs for broilers

Amino Acid Digestibility Methods • PSA 2008 and PSA 2009 – Differences higher than 5 to 15% in AA digestibility without statistical differences • So, how to evaluate effects of modifiers that can improve AA digestibility in that range ? – Precision Fed Rooster Assay – Low Feed Intake – Standardized Ileal Assay – Semi Purified Diets

Objective • This study tested the ability of a commercially available protease to improve the protein and amino acid digestibility of Corn

Material and Methods • Metabolism Trial • 160 Male Cobb 500 • Experimental Design • Experimental Period – 14 to 18 d old adaptation period – 19 to 21 d old total excrete collection – 4 treatments – 8 replicates of 5 birds each • Analyses Protease (PROT/kg) Tr. Ingredients 1 Reference Diet 0 2 Reference Diet 15000 3 60% Reference Diet + 40% Corn 0 4 60% Reference Diet + 40% Corn 15000 – Samples were freezedried – Amino Acids analyzed by HPLC (White et al.. 1986; Hagen et al.. 1989).

Material and Methods • Calculate the Total Apparent Amino Acid Digestibility: – TAAA (TD or RD) = ingested AA– excreted AA ingested AA – TAAA (Ingredient)= AARD + (AATD – AARD) g/g substitution Where: TD – Test Diet; RD – Reference Diet; AARD – Amino Acid in Reference Diet; AATD – Amino Acid in Test Diet. • Data analyzed according to SAS procedures (2001). – Student Test (P<0.10)

Material and Methods • Product Specifications – RONOZYME ProAct is a mono component protease with 75000 PROT/g originated from Nocardiopsis prasina and produced by the bacteria Bacillus licheniformis – Recommend Dose 15000 PROT/kg = 200 ppm – One ProAct activity unit (PROT) is the amount of enzyme that releases 1 µmol of p-nitroaniline from 1 mM substrate (Suc-AlaAla-Pro-Phe-pNA) per minute at pH 9.0 and 37 °C.

Material and Methods • Reference Diet Composition Ingredients Corn SBM Fat DCP 24/18 Limestone Salt Mineral Premix1 Vitamins Premix1 DL-Methionine 99% L-Lysine 78% L-Threonine 98% Other Additives Choline Chloride 70% Total % 53.749 38.747 3.283 2.151 0.951 0.457 0.100 0.100 0.247 0.063 0.007 0.088 0.057 100.000 Calculate Content AME. Kcal 2980 CP. % 22.00 Ca. % 1.00 Av. P. % 0.50 Na. % 0.20 Dig Lys. % 1.15 Dig TSAA. % 0.85 Dig Thr. % 0.76

Material and Methods • Corn Composition Nutrient Fat (%) Moisture (%) Crude Protein (%) 3.59 11.46 8.02 Amino Acids Aspartic Acid Glutamic Acid Serine Glycine Histidine Arginine Threonine Alanine Proline Tyrosine Valine Methionine Cystine Isoleucine Leucine Phenylalanine Lysine Total amino acids % 0.53 1.61 0.42 0.33 0.26 0.42 0.28 0.64 0.76 0.34 0.42 0.21 0.15 0.30 1.06 0.41 0.28 8.43

Material and Methods • Corn Composition Amino Acids Aspartic Acid Glutamic Acid Serine+Glycine Histidine Arginine Threonine Alanine Proline Tyrosine Valine Methionine Cystine Isoleucine Leucine Phenylalanine Lysine Rostagno et al., 2005 0.73 0.26 0.39 0.32 0.29 0.40 0.17 0.19 0.29 1.02 0.41 0.24 % 0.53 1.61 0.75 0.26 0.42 0.28 0.64 0.76 0.34 0.42 0.21 0.15 0.30 1.06 0.41 0.28

Material and Methods •Broiler raised in floor pens until be transferred to batteries •Receiving the same feed w/out enzyme

Material and Methods Metabolism Room • Room of 90 m2 • Controlled environment

Results • Protease effect on Total Apparent Amino Acid Digestibility of Corn / Essential Amino Acids Amino Acids Digestibility Coefficient, % CV P Without Protease With Protease Arginine 81.45 83.56 4.20 0.4211 Threonine 72.16 79.29 3.18 0.0580 Valine 81.41 84.71 2.43 0.0606 Methionine 87.31 87.48 1.64 0.8722 Cystine 75.01 79.19 1.98 0.0081 Isoleucine 82.23 85.22 1.13 0.0042 Leucine 88.33 89.48 0.93 0.0960 Phenylalanine 87.08 88.14 0.84 0.0891 Lysine 87.81 88.02 0.72 0.6483 Histidine 85.52 86.02 1.02 0.4546 Total AA 82.25 84.75 0.91 0.0036

Results • Protease improvement on Total Apparent AA Digestibility of Corn Improvement (% Control) – Essential Amino Acids 120% Mean = 3.18% improvement 115% * 110% * * 105% * 100% His Arg * = P≤0.10 Thr Val Met Cys Ile * * Leu Phe Lys

Results • Protease effect on Total Apparent Amino Acid Digestibility of Corn – Non Essential Amino Acids Amino Acids Digestibility Coefficient, % Without Protease With Protease CV P Aspartic Acid 84.48 84.64 2.17 0.9104 Glutamic Acid 90.66 91.79 1.32 0.2322 Serine 79.51 83.26 1.72 0.0090 Glycine 65.96 73.68 4.46 0.0128 Alanine 79.35 83.81 4.78 0.1572 Proline 82.45 85.98 1.81 0.0170 Tyrosine 86.93 87.84 1.08 0.2246

Results • Protease improvement on Total Apparent AA Digestibility of Corn 120% Mean = 3.4% improvement 115% * 110% * 105% * * * * * * = P≤0.10 * * Le u Ph e Ly s Ile G lu Se r G ly H is A rg Th r A la Pr o Ty r Va l M et C ys 100% A sp Improvement (% Control) – All Amino Acids

Results True AA digestibility CP Lys Met Cis Thr Arg Ile Leu Val His Phe Lemme, 2004 90 92 94 87 85 93 95 94 92 95 94 Rostagno, 2005 87 85,9 92,3 87,9 83,8 92 90,6 95,4 88,2 93,3 91,5 Total Apparent AA digestibility Corn 83,05 87,80 87,30 75,01 72,16 81,45 82,33 88,33 81,41 85,52 87,08 Corn + Protease 83,58 88,02 87,48 79,19 79,29 83,56 85,22 89,48 84,71 86,02 88,14

Conclusion • Using 200 ppm of RONOZYME® ProAct effectively increased utilization of amino acids in Corn on an average of 3.18%.

Acknowledgment • UFLA • NECTA • DSM Nutritional Products

Visit DSM at: • DSM Animal Nutrition and Health

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