The Microscope main ppt

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Information about The Microscope main ppt

Published on September 5, 2010

Author: aditya0291


The Microscope : The Microscope The History : The History Many people experimented with making microscopes Was the microscope originally made by accident? (Most people were creating telescopes) The first microscope was 6 feet long!!! The Greeks & Romans used “lenses” to magnify objects over 1000 years ago. The History : The History Hans and Zacharias Janssen of Holland in the 1590’s created the “first” compound microscope Anthony van Leeuwenhoek and Robert Hooke made improvements by working on the lenses Anthony van Leeuwenhoek 1632-1723 Robert Hooke 1635-1703 Hooke Microscope Slide 4: Scale How a Microscope Works : How a Microscope Works Convex Lenses are curved glass used to make microscopes (and glasses etc.) Convex Lenses bend light and focus it in one spot. How a Microscope Works : How a Microscope Works Ocular Lens (Magnifies Image) Objective Lens (Gathers Light, Magnifies And Focuses Image Inside Body Tube) Body Tube (Image Focuses) Bending Light: The objective (bottom) convex lens magnifies and focuses (bends) the image inside the body tube and the ocular convex (top) lens of a microscope magnifies it (again). The Parts of a Microscope : The Parts of a Microscope Slide 8: Body Tube Nose Piece Objective Lenses Clips Diaphragm Light Source Stage Arm Stage Coarse Adj. Fine Adjustment Base Skip to Magnification Section Ocular Lens Body Tube : Body Tube The body tube holds the objective lenses and the ocular lens at the proper distance Diagram Nose Piece : Nose Piece The Nose Piece holds the objective lenses and can be turned to increase the magnification Diagram Lenses and the Bending of Light : Lenses and the Bending of Light light is refracted (bent) when passing from one medium to another refractive index a measure of how greatly a substance slows the velocity of light direction and magnitude of bending is determined by the refractive indexes of the two media forming the interface Lenses : Lenses focus light rays at a specific place called the focal point distance between center of lens and focal point is the focal length strength of lens related to focal length short focal length more magnification Objective Lenses : Objective Lenses The Objective Lenses increase magnification (usually from 10x to 40x) Diagram Microscopy: The Instruments : Microscopy: The Instruments Refractive index: The light-bending ability of a medium. The light may bend in air so much that it misses the small high-magnification lens. Immersion oil is used to keep light from bending. Figure 3.3 Ocular Lens/Eyepiece : Ocular Lens/Eyepiece Magnifies the specimen image Diagram Stage Clips : Stage Clips These 2 clips hold the slide/specimen in place on the stage. Diagram Diaphragm : Diaphragm The Diaphragm controls the amount of light on the slide/specimen Turn to let more light in or to make dimmer. Diagram Light Source : Light Source Projects light upwards through the diaphragm, the specimen and the lenses Some have lights, others have mirrors where you must move the mirror to reflect light Diagram Arm : Arm Used to support the microscope when carried. Holds the body tube, nose piece and objective lenses Diagram Stage : Stage Supports the slide/specimen Diagram Coarse Adjustment Knob : Coarse Adjustment Knob Moves the stage up and down (quickly) for focusing your image Diagram Fine Adjustment Knob : Fine Adjustment Knob This knob moves the stage SLIGHTLY to sharpen the image Diagram Base : Base Supports the microscope Diagram Magnification : Magnification To determine your magnification…you just multiply the ocular lens by the objective lens Ocular 10x Objective 40x:10 x 40 = 400 Objective Lens have their magnification written on them. Ocular lenses usually magnifies by 10x So the object is 400 times “larger” Microscope Resolution : Microscope Resolution ability of a lens to separate or distinguish small objects that are close together wavelength of light used is major factor in resolution shorter wavelength  greater resolution Slide 30: working distance distance between the front surface of lens and surface of cover glass or specimen Types of The Light Microscope : Types of The Light Microscope many types bright-field microscope dark-field microscope phase-contrast microscope fluorescence microscopes are compound microscopes image formed by action of 2 lenses Using a Microscope : Using a Microscope Start on the lowest magnification Don’t use the coarse adjustment knob on high magnification…you’ll break the slide!!! Place slide on stage and lock clips Adjust light source (if it’s a mirror…don’t stand in front of it!) Use fine adjustment to focus Can you name the parts of a compound microscope? : Can you name the parts of a compound microscope? Answers : Answers base  mirror (light source)   diaphragm   stage   stage clips low power objective lens   high power objective lens   nosepiece   arm  fine focus knob   body tube   coarse focus knob   eyepiece

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