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Sym06ppt Oram

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Published on October 29, 2007

Author: FunnyGuy

Source: authorstream.com

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Slide1:  Stem Cell Technology Certificate Program City College of San Francisco Director: Phil Jardim Instructor: Shane Oram, PhD. Associate Investigator UCSF-VA Medical Center Slide2:  City College of San Francisco announces ground-breaking Stem Cell Research Technician Certificate program SAN FRANCISCO, California, April 13, 2005 — San Francisco Mayor Gavin Newsom, City College of San Francisco (CCSF) Board President Rodel E. Rodis, Board Vice President Dr. Natalie Berg, and Chancellor Dr. Philip R. Day, Jr., announced today the receipt of a $779,067 grant under the California Governor’s Funding category, Growth Industries – High Wage, High Skill Job Training. This two-year grant will enable City College to create a Stem Cell Research Technician Certificate program which will equip participants with the advanced skills needed to find jobs in the lucrative and rapidly growing field of stem cell research. Slide3:  Benefits of WIA Job Seekers Universal access to job search and labor market information Advice, counseling, and support Education and skills training Individual choice of service Employers Influence over local area employment policy Improved and trained employee pool Development of on-the-job and customized training opportunities Assistance for laid-off workers Community Access to local area job market information Improved workforce quality Services designed for local area needs Reduced need for welfare WIA – Workforce Investment Act Slide4:  Stem Cell Technology Certificate Program How to get program started? What SKILLS are needed to allow students (undergraduate level) who complete program to be prepared for a career in Stem Cell Biology? What CLASSES are need to teach these skills? EQUIPMENT??? Establish COLLABORATIONS with UCSF – Dr. Mort Cowan - Director of Pediatric Bone Marrow Transplant (UCSF)) Dr. Renee Reijo-Pera - Co-Director, Program in Human Stem Cell Biology (UCSF) Dr. Bruce R. Conklin - Gladstone Institute of Cardiovascular Disease (UCSF) 4. Establish COLLABORATIONS WITH INDUSTRY… Miltenyi Biotec, others… 5. Set up internship program for students that complete certificate program Slide5:  Stem Cell Technology Certificate Program 3 Courses: BTEC21A: Basic Mammalian Cell Culture (6 weeks/ 9 hours per week) Text – Culture of Animal Cell: A manual of basic technique (R. Ian Freshney, 5th Edition) BTEC21B: Fluorescent Cell Technology (6 weeks/ 9 hours per week) BTEC21C: Stem Cell Technology (8 weeks/ 9 hours per week) Text – Essentials of Stem Cell Biology (Lanza, 1st Edition) Minimum prerequisites for BTEC 21A: One semester of college-level general biology with lab and two semesters of college-level general chemistry including organic with lab. Courses offered in the Fall, Spring, and Summer. Day and Night Classes. Students with proper background are capable of completing program in 1 calendar year. Slide6:  MODEL: Xeroderma pigmentosum (XP) – is a rare autosomal recessive disease of humans characterized by an accelerated chronic degeneration of sun−exposed areas of the body, including an elevated risk of developing cancers of the skin. 3 Cells lines: Chinese Hamster Ovary (CHO) Cells AA8 – Wild type, functioning Nucleotide Excision Repair (NER) UV5 – NER deficient (sensitive to UV irradiation) 5P2E – NER restored by transfection Cells and technical assistance provided by: Dr. James Cleaver - Professor of Dermatology and Pharmaceutical Chemistry (UCSF) BTEC21A: Basic Mammalian Cell Culture (6 weeks/ 9 hours per week) Slide7:  SKILLS: Understanding of cell culture techniques and various components such as fetal calf serum, growth factors, and antibiotics Lab Notebook Management - Inventory supplies and media components Pipetting 101 – Proper use of micropipettes and serological pipettes Aseptic technique in a laminar flow hood Operation and maintenance of C02 incubators, water baths, and inverted microscope Count cells using a hemacytometer - Compare cell growth under varying conditions BTEC21A: Basic Mammalian Cell Culture (6 weeks/ 9 hours per week) Slide8:  SKILLS: (Continued) Plate cells at equal density - calculate plating efficiency, cell density and construct growth curves Calculate, predict, and prepare proper cell concentrations and make appropriate transfers and feeding Introduction to experiment design and execution Perform experiment utilizing normal and mutant cell lines and graph their survival rates after UV-induced and chemically induced DNA damage Introduction to writing a scientific publication - record, analyze, and present qualitative and quantitative data. BTEC21A: Basic Mammalian Cell Culture (6 weeks/ 9 hours per week) Slide9:  MODEL: CHO and COS7 cells SKILLS: Use of Coulter counter to count cells Describe different types and applications of fluorophores used in cell biology research. Demonstrate proper use and care of a fluorescent microscope. Perform nucleic acids staining with PI Perform organelle staining with various fluorophores. BTEC21B: Fluorescent Cell Technology (6 weeks/ 9 hours per week) Slide10:  SKILLS: (Continued) Perform transfections using fluorescent markers Analyze transfected cells using fluorescent microscopy. Identify subcellular localization – MitoTracker, LysoTracker, GFP-nuclear, etc. Perform immunostaining and apoptosis assays. Discuss current applications of fluorescent technology, DNA microarrays, confocal microscopy and FACS analysis. BTEC21B: Fluorescent Cell Technology (6 weeks/ 9 hours per week) Slide11:  LECTURE COMPONENT: Ethics of stem cell biology and cloning Introduction to stem cell biology, self renewal, and pluripotency ES cells and primordial germ cells in early development Adult stem cells and research applications Hematopoietic stem cells and bone marrow transplants Mesenchymal stem cells Uses of stem cells in regenerative medicine Vascular endothelial precursors and wound healing Neural stem cells and restorative neurobiology Pancreatic stem cells and diabetes Liver regeneration and stem cells Cardiology and stem cells BTEC21C: Stem Cell Technology (8 weeks/ 9 hours per week) Slide12:  BTEC21C: Stem Cell Technology (8 weeks/ 9 hours per week) LABORATORY COMPONENT: 1) Isolation of stem cells from adult mouse – Dr. Mort Cowan – Director of Pediatric Bone Marrow Transplant (UCSF) Isolation of hematopoietic and mesenchymal cells from mouse bone marrow (Miltenyi Biotec Products) 2) Introduction and basics of flow cytometry – Analysis of cells using a BD FACScan 3) Culturing mouse ES cells – Dr. Bruce Conklin – Gladstone Institute of Cardiovascular Disease (UCSF) Growing feeder cells and ES cells while maintaining their pluripotency 4) Preparing ES cell karyotypes – Preparing/Analyzing chromosome spreads 5) Differentiation of ES stem cells – Hanging drop technique for differentiation of ES cells Use of antibodies and immunostaining to confirm differentiation of ES cells Slide13:  BTEC21C: Stem Cell Technology (8 weeks/ 9 hours per week) SKILLS: Relate principles of stem cell biology to proper laboratory techniques. Critically and independently assess the reality and future of applied stem cell biology. Differentiate between different types of stem cells in terms of their function, use and care. Isolate mesenchymal and hematopoietic stem cells from mouse bone marrow. Isolate c-kit positive hematopoietic stem cells Describe the underlying principles of flow cytometry. Use a BD FACScan (flow cytometry). Analyse cell surface markers and cell types using a FACScan. Slide14:  SKILLS: (Continued) Handle and care for mouse embryonic stem (ES) cells while maintaining their pluripotency. Properly culture and maintain feeder cells. Use proper culture techniques to induce differentiation of ES cells into different cell types. Perform karyotyping on ES cells. Properly freeze and thaw stem cells to maintain viability. BTEC21C: Stem Cell Technology (8 weeks/ 9 hours per week) Slide15:  Acknowledgements CCSF Director: Phil Jardim Kristin Hershbell Dr. Carin Zimmerman Dr. Edie Leonhardt Dr. Wing Tsao Dr. Carol Toebe Dr. Elaine Johnson UCSF Dr. Mort Cowan Ms. Elizabeth Dunn Dr. Renee Reijo-Pera Dr. Bruce Conklin Miltenyi Biotech Mr. Thad Baker

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