Steps of Glycolysis in a brief

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Information about Steps of Glycolysis in a brief

Published on February 15, 2014

Author: Hillolvai



Here i made a presentation on the topic GLYCOLYSIS. I Demonstrated the ten steps of Glycolysis in a few word and flow chart so that you can easily learn about it.

Glycolysis Rahman R Hillol KGHS,Dhaka.

What is Glycolysis Glycolysis (from the Greek glykos, meaning sweet, and lysis, meaning splitting) involves the breakdown of glucose, a simple sugar. This process can occur in the presence or absence of oxygen, that is, under aerobic or anaerobic conditions.

History of Glycolysis During the 1930s, the efforts of several German biochemists, including Gustav Embden, Otto Meyerhof, and Jacob Parnas, determined that glycolysis involves 10 steps, each one catalyzed by a different enzyme.

Concept of Glycolysis Glycolysis

Step 1 Glucose is phosphorylated by ATP. Glucose-6-phosphate is more easily trapped in the cell compared to glucose. ATP ADP Hexokinase Glucose Glucose-6-Phosphate

Step 2 The structure of glucose-6-phosphate is rearranged to fructose-6-phosphate. Phosphogluco isomerase Glucose-6-Phosphate Fructose-6-phosphate

Step 3 Fructose-6-phosphate is phosphorylated to make fructose-1,6-bisphosphate. ATP ADP Phosphofructo kinase Fructose-6-phosphate Fructose-1,6-bisphosphate

4. Fructose-1,6-bisphosphate is cleaved into dihydroxyacetone phosphate and Dihydroxyacetone phosphate glyceraldehyde-3-phosphate. 5. Di-hydroxyl-acetone phosphate is somerized to glyceraldehyde-3-phosphate. 4. 5. Isomerase Aldolase Fructose-1,6-bisphosphate Glyceraldehyde-3phosphate (x2)

6. Glyceraldehyde-3-phosphate is oxidized to 1,3bisphosphoglycerate. NADH is produced. In 1,3-bisphospho-glycerate,the phosphate group in the upper left is destabilized, meaning that the bond will break in a highly exergonic reaction. Unstable Phosphate Bond 2NADH 2 NAD+ Glyceraldehyde-3phosphate (x2) +2H+ Glyceraldehyde-3phosphate dehydrogenase 1,3-bisphosphoglycerate (x2)

7. A phosphate is removed from 1,3-bis-phospho-glycerate to form 3-phosphor-glycerate. The removed phosphate is transferred to ADP to make ATP via substrate-level phosphorylation. Unstable Phosphate Bond 2ATP 2ADP Phosphoglycero– kinase 1,3-bisphosphoglycerate (x2) 3-phosphoglycerate(x2)

8. The phosphate group in 3 phosphoglycerate is moved to a new location, creating 2phosphoglycerate Phosphoglycero– mutase 3-phosphoglycerate(x2) 2-phosphoglycerate(x2)

9. A water molecule is removed from 2-phosphor-glycerate to form phosphor-enol-pyruvate. In phosphor-enol-pyruvate, the phosphate group is destabilized, meaning that the bond will break in a highly exergonic reaction. Unstable phosphate bond Enolase 2-phosphoglycerate(x2) Phosphoenolpyruvate(x2)

10. A phosphate is removed from phosphor-enol-pyruvate to form pyruvate. The removed phosphate is transferred to ADP to make ATP via substrate-level phosphorylation. Unstable phosphate bond 2ADP 2ATP Pyruvate Kinase Phosphoenolpyruvate(x2) pyruvate(x2)

:p Thanks God! It’s over at last!!

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