Slicing Brains Very Quickly - Todd Huffman

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Information about Slicing Brains Very Quickly - Todd Huffman
Technology

Published on May 3, 2009

Author: IgnitePhoenix

Source: slideshare.net

Description

Video: http://ignitephoenix.blip.tv/file/1839013/

Event: Ignite Phoenix 3 @ TCA
Event Date: February 25th, 2009

I’ve been an entrepreneur for the last few years, but I’d like to tell you about my real passion in life — slicing brains into very thin slices (very quickly), and taking pictures. High-throughput quantitative analysis of neural morphology and connectivity enables awesome insights into human thought. I’ll show lots of pretty pictures and tell dazzling stories.

The Mind, seen in Slices 100 billion neurons in the brain ๏ Each neuron with 7 thousand synapses Phoenix 700,000,000,000,000 Connections Press play.... Out of this ๏ emerges our consciousness We’re trying to ๏ observe this structure...

We being... KESM data from David Mayerich at TAMU ๏ Brain Networks Lab under Dr Choe Phoenix www.research.cs.tamu.edu/bnl - ATLUM data from Kenneth Hayworth & ๏ 'Bobby' Kasthuri in Lichtman Laboratory at Harvard www.mcb.harvard.edu/lichtman/ATLUM/ATLUM_web.htm - All images are belong to the respective labs ๏ and are used with permission

Road to 3D starts in 2 Phoenix

Knife Edge Scanning Microscope (KESM) Phoenix

Step 1: Slice and Image Phoenix

Step 2: Reconstruct Phoenix

Phoenix

Phoenix

Phoenix

Automatic Tape-Collecting Lathe Ultramicrotome (ATLUM) Phoenix

Step 1: Spool brain on to tape Phoenix

Step 2: Index and store brain Phoenix

Step 3: Take Picture in SEM Phoenix

Step 4: Reconstruct Phoenix

Phoenix Zo ooo oo oo oo m

Phoenix

Phoenix THIS IS YOUR MIND

Phoenix

Perspective 1/50,000th of the way completed ๏ Phoenix but... ๏ Engineering problems are solvable ๏ High-throughput reconstruction of neural ๏ morphology and connectivity is going to do to neuroscience what the human genome project has done to genetics

Call to Action What’s required to solve the problem... ๏ Phoenix Computer Science ๏ Biology ๏ Engineering ๏ Politics & Policy ๏ Media ๏ Fund Raising ๏ Entrepreneurs ๏

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