ReaMetrix Overview

33 %
67 %
Information about ReaMetrix Overview
Health & Medicine

Published on February 18, 2009

Author: zmanian

Source: slideshare.net

Description

ReaMetrix Overview Presentation

ReaMetrix An Introduction

Vision ReaMetrix is committed to become a leading supplier of high quality and affordable integrated diagnostic solutions to resource poor settings, by leveraging local resources innovatively.

ReaMetrix is committed to become a leading supplier of high quality and affordable integrated diagnostic solutions to resource poor settings, by leveraging local resources innovatively.

ReaMetrix at a glance Life science technology company founded in May, 2003, CEO - Dr. Bala Manian, Founder of many life science companies Strong management team with global experience in process and product development in regulated environments. Started in San Carlos, CA with the centre of activity in Bangalore, India. State of the art lab and production facility in Bangalore India. Strong team, 42 employees, 38 in India, 15 with PhD’s. ISO 9001:2000 Certified in May 2008 SAN CARLOS (HEAD OFFICE) BANGALORE (MANUFACTURING & R&D FACILITY) SINGAPORE (REPRESENTATIVE OFFICE)

Life science technology company founded in May, 2003, CEO - Dr. Bala Manian, Founder of many life science companies

Strong management team with global experience in process and product development in regulated environments.

Started in San Carlos, CA with the centre of activity in Bangalore, India.

State of the art lab and production facility in Bangalore India.

Strong team, 42 employees, 38 in India, 15 with PhD’s.

ISO 9001:2000 Certified in May 2008

Expertise in Gene design Cloning Expression Amplification & sequencing Expertise in Tailoring assays to hardware platform Cell based and Bead based assays ELISA, FLISA From Proof of Concept to supply of assay kits Expertise in Labeling of proteins and nucleic acids with Organic dyes Phycobiliproteins Nanocrystals Expertise in Optics based instrumentation and detection systems. Integrated solutions with reagents to solve life science problems Assay Development Stable Cell Lines Custom Conjugations Instrumentation ReaMetrix Competencies Core competencies drive Diagnostics & Research businesses

Expertise in

Gene design

Cloning

Expression

Amplification & sequencing

Expertise in

Tailoring assays to hardware platform

Cell based and Bead based assays

ELISA, FLISA

From Proof of Concept to supply of assay kits

Expertise in

Labeling of proteins and nucleic acids with

Organic dyes

Phycobiliproteins

Nanocrystals

Expertise in

Optics based instrumentation and detection systems.

Integrated solutions with reagents to solve life science problems

State of the art Indian Facility

Driving Value Through Innovation The ReaMetrix Experience PE TR Cy5 Cy5.5 Cy7

Immunofluorescent Conjugates Identifying bright & affordable fluorophores Optimizing process chemistry to generate high performance conjugates Innovation in design and process

Identifying bright & affordable fluorophores

Optimizing process chemistry to generate high performance conjugates

Identifying Alternate Dyes – Developing a Palette Phycoerythrin and the range of dyes in the red region PE emits at 562 nm Texas Red or equivalents emit at 610 nm Cy5,Dy649, Hilyte647 emit at 670 nm Cy5.5, Hilyte680, Dy680 emit at 700 nm Cy7 or Hilyte750, Dy750 emit at 770 nm PE TR Cy5 Cy5.5 Cy7 Allophycocyanin and the range of dyes in the red region APC emits at 660 nm Cy5.5 or equivalents emit at 700 nm Alexa700 or LiCor700 emit at 720 nm Cy7 or Hilyte750, Dy750 emit at 770 nm Licor800 or equivalents emit at 800 nm APC Cy5.5 A700 Cy7 Licor800 High Quality Fluorescent Dyes at Affordable Cost

Phycoerythrin and the range of dyes in the red region

PE emits at 562 nm

Texas Red or equivalents emit at 610 nm

Cy5,Dy649, Hilyte647 emit at 670 nm

Cy5.5, Hilyte680, Dy680 emit at 700 nm

Cy7 or Hilyte750, Dy750 emit at 770 nm

Allophycocyanin and the range of dyes in the red region

APC emits at 660 nm

Cy5.5 or equivalents emit at 700 nm

Alexa700 or LiCor700 emit at 720 nm

Cy7 or Hilyte750, Dy750 emit at 770 nm

Licor800 or equivalents emit at 800 nm

Developing Immunofluorescent Conjugates Optimize yields for fluorescent conjugations using process chemistry Incoming Quality Control Acquire unlabeled antibodies & other reagents from third parties Component purification Functional validation Unitizing and packaging Deliver finished product to customer Specification/ Quality Assurance Purification Process quality control testing Conjugation Process is our Product

Optimize yields for fluorescent conjugations using process chemistry

Key features of ReaMetrix conjugates Site Specific Labeling of Antibodies with precise Dye to Protein ratios High-quality (certificate of analysis provided for each product with all the requisite specifications) Validated for fluorescence intensity, activity, protein & dye concentration, dye integrity, emission spectra Perform functional validation where required Audit trail (each and every step is documented to enable a high degree of transparency) Selected Customers who have validated ReaMetrix Conjugates Harvard University, Duke University, National Institute of the Health, USA, Applied Biosystems Tata Memorial Hospital

Site Specific Labeling of Antibodies with precise Dye to Protein ratios

High-quality (certificate of analysis provided for each product with all the requisite specifications)

Validated for fluorescence intensity, activity, protein & dye concentration, dye integrity, emission spectra

Perform functional validation where required

Audit trail (each and every step is documented to enable a high degree of transparency)

Selected Customers who have validated ReaMetrix Conjugates

Harvard University,

Duke University,

National Institute of the Health, USA,

Applied Biosystems

Tata Memorial Hospital

Development conjugates for Leukemia-Lymphoma Developed with 3 colors so that it can be used in India where 3 to 4 color Flow cytometers are prevalent. Conjugates have been validated on peripheral blood/bone-marrow samples or stable cell lines with receptors. The performance of these conjugates has been benchmarked with existing commercially available conjugates ReaMetrix Conjugates can be provided at smaller unit sizes so that they are affordable and accessible to more testing centers

Developed with 3 colors so that it can be used in India where 3 to 4 color Flow cytometers are prevalent.

Conjugates have been validated on peripheral blood/bone-marrow samples or stable cell lines with receptors.

The performance of these conjugates has been benchmarked with existing commercially available conjugates

ReaMetrix Conjugates can be provided at smaller unit sizes so that they are affordable and accessible to more testing centers

Products Developed at ReaMetrix Reagents for enumerating CD3+CD4+ reagents TriTStat CD3/CD4/CD8 TriTStat CD3/CD4/CD45 ReaPan 34845 ReaTcount ReaPan B27 – Reagent for typing HLA B27 ReaPan S34 – Reagent for enumerating CD34+ Hematpoietic stem cells Value addition through Innovation

Reagents for enumerating CD3+CD4+ reagents

TriTStat CD3/CD4/CD8

TriTStat CD3/CD4/CD45

ReaPan 34845

ReaTcount

ReaPan B27 – Reagent for typing HLA B27

ReaPan S34 – Reagent for enumerating CD34+ Hematpoietic stem cells

Typical Characteristics of ReaMetrix Products Antibodies used are workshop approved clones Fluorescent labels used have no intellectual property entanglements. The assay protocols associated with these tests are similar to commercially existing assays The major difference is that ReaMetrix reagents are delivered in a unitized dry format. Particularly in resource poor settings, the dry reagent format reduces the cost of performing the assay and opens up additional options for providing access to the test for the patients.

Antibodies used are workshop approved clones

Fluorescent labels used have no intellectual property entanglements.

The assay protocols associated with these tests are similar to commercially existing assays

The major difference is that ReaMetrix reagents are delivered in a unitized dry format.

Particularly in resource poor settings, the dry reagent format reduces the cost of performing the assay and opens up additional options for providing access to the test for the patients.

Reagent for monitoring HIV progression through the Enumeration of CD3+CD4+ Cells TriTStat CD3/4/8 and CD3/4/45 MAH CD4 antibody labeled w/ Atto 488 dye (FITC alternative) MAH CD8 antibody (or MAH CD45) antibody labeled with R-Phycoerythrin (PE) MAH CD3 antibody labeled w/ PE-Dyomics 649 (Cy5 alternative) tandem dye Precise number of fluorescent beads Stabilizers Tests are suitable to run on open flow cytometers such as BD FACScan, BD FACSCalibur, Coulter EPICS etc . Reagents validated at YRG care, Chennai NARI, Pune TriTStat CD3/4/8 is CE marked Now available as a Four color reagent as ReaPan 34845 Reagent supplied in Dry, Unitized Format No cold chain Up to 5 day stability of stained-fixed samples CD4 0 300 600 900 1200 1500 0 300 600 900 1200 1500 Rmx Dry T-STAT Coulter TetraChrome r = 0.994

TriTStat CD3/4/8 and CD3/4/45

MAH CD4 antibody labeled w/ Atto 488 dye (FITC alternative)

MAH CD8 antibody (or MAH CD45) antibody labeled with R-Phycoerythrin (PE)

MAH CD3 antibody labeled w/ PE-Dyomics 649 (Cy5 alternative) tandem dye

Precise number of fluorescent beads

Stabilizers

Tests are suitable to run on open flow cytometers such as BD FACScan, BD FACSCalibur, Coulter EPICS etc .

Reagents validated at

YRG care, Chennai

NARI, Pune

TriTStat CD3/4/8 is CE marked

Now available as a Four color reagent as ReaPan 34845

Reagent supplied in Dry, Unitized Format

No cold chain

Up to 5 day stability of stained-fixed samples

ReaTcount – Affordable Alternative to FACScount ™ Reagent Supplied in a Dry, Unitized Format Reagent validated at National HIV Immunology Laboratory, Canada Zimbabwe Excellent Correlation

Reagent Supplied in a Dry, Unitized Format

Reagent validated at

National HIV Immunology Laboratory, Canada

Zimbabwe

ReaPan B27 – For typing HLA B27 Use 2 clones – enhances sensitivity and specificity Selection of CD3+ population to reduce false positives Choice of PE as the dye to identify the HLA B27 population – High fluorescence Addition of unconjugated Anti B7 antibody reduces nonspecific binding Provision of Fluorescence Intensity beads in each reagent tube provides a built-in quality/reference standard Dry Unitized Format Simplified work flow

Use 2 clones – enhances sensitivity and specificity

Selection of CD3+ population to reduce false positives

Choice of PE as the dye to identify the HLA B27 population – High fluorescence

Addition of unconjugated Anti B7 antibody reduces nonspecific binding

Provision of Fluorescence Intensity beads in each reagent tube provides a built-in quality/reference standard

Dry Unitized Format

Simplified work flow

ReaPan S34 – For Enumerating CD34 cells Convenience: Dry, unitized ready to test format. Single platform enumeration using Modified ISHAGE guidelines. Stability: Dried down reagent does not require Cold Chain Maintenance. Workflow: Simple accurate and repeatable Lyse no wash protocol. Differentiates viable CD34+ stem cells from Non Viable(7-AAD). Single step pipetting : Minimizes errors.

Convenience: Dry, unitized ready to test format.

Single platform enumeration using Modified ISHAGE guidelines.

Stability: Dried down reagent does not require Cold Chain Maintenance.

Workflow: Simple accurate and repeatable Lyse no wash protocol.

Differentiates viable CD34+ stem cells from Non Viable(7-AAD).

Single step pipetting : Minimizes errors.

New Product Ideas Under development in our labs

ReaPanMicro – Presence/Absence Test for Bacteria in Urine A diagnostic test to confirm the absence of bacteria in urine, based on the principle of nucleic acid detection in bacteria. Key Features of the Test High sensitivity ( 100% Negative Predictive Value ) Time to Result: Within 15 min of sample reaching a flow cytometer Easy to use, simple assay workflow Culture Negative Culture Positive 146 Samples By Culture 67 22 TN 100.0% NPV 72.2% PPV 0 Negative by Flow 57 Positive by Flow TP Overall

A diagnostic test to confirm the absence of bacteria in urine, based on the principle of nucleic acid detection in bacteria.

Key Features of the Test

High sensitivity ( 100% Negative Predictive Value )

Time to Result: Within 15 min of sample reaching a flow cytometer

Easy to use, simple assay workflow

Bacteria Speciation Using Fluorescent Tags Using Bacteriophages to detect bacteria A: Bright field image of S.aureus bacteria stained with tag protein B : Fluorescence image of stained S. aureus bacteria stained with tag protein C: Bright field image of E. coli bacteria that was incubated with tag protein D: Fluorescence image of E. coli bacteria that was incubated with tag protein A B C D

Using Bacteriophages to detect bacteria

A: Bright field image of S.aureus bacteria stained with tag protein

B : Fluorescence image of stained S. aureus bacteria stained with tag protein

C: Bright field image of E. coli bacteria that was incubated with tag protein

D: Fluorescence image of E. coli bacteria that was incubated with tag protein

Products under development - Controls Positive Control Wells for Malaria In collaboration with Foundation for Innovation in Diagnostics (FIND). To develop as a positive controls that will validate the efficacy of Rapid Diagnostic tests for Malaria Blood Controls To develop blood controls for use in flow cytometry http://www.finddiagnostics.org/news/newsletters/issue_10-jun_08/page03.shtml Dot plots of 20 day stabilized blood samples

Positive Control Wells for Malaria

In collaboration with Foundation for Innovation in Diagnostics (FIND).

To develop as a positive controls that will validate the efficacy of Rapid Diagnostic tests for Malaria

Blood Controls

To develop blood controls for use in flow cytometry

Products under development ReaPan Leuco – To enumerate Leukocytes in Leukoreduced Platelets For rare event detection and enumeration of leukocytes HbA1C An affordable method for %HbA1C using immunofluorescence Viral vs Bacterial Pyrexia Differentiating between Viral and Bacterial Fever Leukocytes Beads

ReaPan Leuco – To enumerate Leukocytes in Leukoreduced Platelets

For rare event detection and enumeration of leukocytes

HbA1C

An affordable method for %HbA1C using immunofluorescence

Viral vs Bacterial Pyrexia

Differentiating between Viral and Bacterial Fever

Products under development ReaPan CD61/41 – For the enumeration of platelets using immunofluorescent staining For use in cases of Thrombocytopenia where existing enumeration methods do not work ReaPan Mono – To enumerate Monocytes in peripheral blood.

ReaPan CD61/41 – For the enumeration of platelets using immunofluorescent staining

For use in cases of Thrombocytopenia where existing enumeration methods do not work

ReaPan Mono – To enumerate Monocytes in peripheral blood.

ReaDev Extending Innovations to Partners

Stable Cell Line Development Example of GPCR expressing cell lines developed at ReaMetrix Stable CHOKI cells expressing IL8RB stained with fluorescently labeled chemokine IL8

Example of GPCR expressing cell lines developed at ReaMetrix

Bead Based Assay Development for US biotechnology Company Develop Sandwich Bead Based assay for three different antigens with lower detection limits of 0.5 ng/mL Transfer assay protocol with prototype kits (100 to 1000 tests/assay) Working assay format with antibody coated carboxy beads and detection through Biotinylated Ab.SAV-PE successfully developed at desired detection limits Delivered complete Antigen Detection Assay kits with detailed report including assay protocols Antibody Screening Screened various commercially available antibodies (Ab) against analyte for best Kd Screened Antibodies in pairs to identify the pair with best detection sensitivity Developed a bead based assay for the chosen analyte Capture Ab on carboxy microsphere bead Biotinylate detection Ab. Tertiary Detection Through SAV-PE Bead Based Assay Format Antibody Pair Selection Assay Development R E A M E T R I X P R O C E S S Project Deliverables

Develop Sandwich Bead Based assay for three different antigens with lower detection limits of 0.5 ng/mL

Transfer assay protocol with prototype kits (100 to 1000 tests/assay)

Working assay format with antibody coated carboxy beads and detection through Biotinylated Ab.SAV-PE successfully developed at desired detection limits

Delivered complete Antigen Detection Assay kits with detailed report including assay protocols

Screened various commercially available antibodies (Ab) against analyte for best Kd

Screened Antibodies in pairs to identify the pair with best detection sensitivity

Developed a bead based assay for the chosen analyte

Capture Ab on carboxy microsphere bead

Biotinylate detection Ab.

Tertiary Detection Through SAV-PE

Expression of cardiovascular protein for MAb development for US Healthcare Company Develop protocol for protein expression in mammalian cells. The protein is to be used for MAb development Delivered purified protein at 1 mg/L expression level on optimized cell line Protein expression and purification in native conformation without tags Expression Site directed mutagenesis Cloning into SK+ vector Sub-cloning into expression vectors Sequencing to confirm the clone Transfection and expression in three mammalian cell lines: CHO K1, COS 7, HEK 293 Affinity chromatography Anion exchange chromatography Gel Filtration Characterization SDS Page for Purity, Western Blot Analysis for Specificity, ELISA for quantitation Transfection Protein Purification & Characterization R E A M E T R I X P R O C E S S Project Deliverables Optimized Promoter/cell combination DNA quantity per cell Lipid to DNA ratio Transfection time Lipids DNA Lipid complex Cloned DNA Secreted Protein

Develop protocol for protein expression in mammalian cells.

The protein is to be used for MAb development

Delivered purified protein at 1 mg/L expression level on optimized cell line

Protein expression and purification in native conformation without tags

Site directed mutagenesis

Cloning into SK+ vector

Sub-cloning into expression vectors

Sequencing to confirm the clone

Transfection and expression in three mammalian cell lines: CHO K1, COS 7, HEK 293

Affinity chromatography

Anion exchange chromatography

Gel Filtration

Characterization

SDS Page for Purity, Western Blot Analysis for Specificity, ELISA for quantitation

Optimized

Promoter/cell combination

DNA quantity per cell

Lipid to DNA ratio

Transfection time

Towards Affordable Diagnostics ReaMetrix Approach

Clinical Information Generation – Current Scenario Sample journey and cost accumulation Acquire Process Run Provide Results Transport Collection centre Path Lab Doctor Hospital Centralized Path Lab Syringe/Vacutainer/Overheads Cold chain/Courier Tech Labor + Reagent Capital Equipment Amortization Lab Infrastructure Overheads CD4 test example: ~10 to 20 times cost addition from reagent manufacture to cost to patient Average cycle time for results ~ 48 to 72 hours Layers of margin at every step

CD4 test example:

~10 to 20 times cost addition from reagent manufacture to cost to patient

Average cycle time for results ~ 48 to 72 hours

Issues with the current scenario Technical Aged blood samples; accuracy & reliability of results Time lag for treatment decisions Lack of a patient centric approach Imported equipment not designed for Indian environment. Eg. Automation, sample aggregation…. Commercial Encourages too many layers of margin affecting affordability Capital costs for imported equipment act as big barrier to entry as well as in adoption of closer to customer models Social Absence of Economic value-add at the local level Local economic activity needs to be promoted for enhanced reach of diagnostics

Technical

Aged blood samples; accuracy & reliability of results

Time lag for treatment decisions

Lack of a patient centric approach

Imported equipment not designed for Indian environment. Eg. Automation, sample aggregation….

Commercial

Encourages too many layers of margin affecting affordability

Capital costs for imported equipment act as big barrier to entry as well as in adoption of closer to customer models

Social

Absence of Economic value-add at the local level

What is the ReaMetrix Solution? Total systems approach – in development Instrument designed for local context  robust & affordable. Reagents developed to suit local conditions  temperature & humidity. Process improvement leveraging local talent  Reducing the cost of the consumable kits. Alternate business/distribution models enabling local empowerment . From a clinical standpoint: Affordability enables more frequent testing & facilitates a more patient-centric approach. Accessibility facilitates rapid transmittal of information at the local level. The ReaScan System – A Total Systems Offering

Total systems approach – in development

Instrument designed for local context  robust & affordable.

Reagents developed to suit local conditions  temperature & humidity.

Process improvement leveraging local talent  Reducing the cost of the consumable kits.

Alternate business/distribution models enabling local empowerment .

From a clinical standpoint:

Affordability enables more frequent testing & facilitates a more patient-centric approach.

Accessibility facilitates rapid transmittal of information at the local level.

ReaScan – In Development

Summary ReaMetrix is a platform for implementing innovative solutions to address the diagnostic needs of India ReaMetrix is keen to work with partners who share a similar philosophy to create new solution opportunities

ReaMetrix is a platform for implementing innovative solutions to address the diagnostic needs of India

ReaMetrix is keen to work with partners who share a similar philosophy to create new solution opportunities

Manufactured by ReaMetrix India Pvt. Ltd. 50-B, II Phase, Peenya Industrial Area Peenya, Bangalore 560058, India Ph: +91-80-28378693/5, Fax: +91-80-41172451 E-mail: info@reametrix.com www.reametrix.com Further details:

Add a comment

Related presentations

Related pages

Working at ReaMetrix | Glassdoor

See what employees say it's like to work at ReaMetrix. Salaries, reviews, and more - all posted by employees working at ReaMetrix.
Read more

ReaMetrix Reviews | Glassdoor

ReaMetrix . Unclaimed Profile. Overview Overview. 2 Reviews--Salaries--Inter­views--Benefits--Jobs. More. Photos; Follow Add a Review. Follow Add a Review.
Read more

Reametrix India Pvt Ltd.: Private Company Information ...

Reametrix India Pvt Ltd. company research & investing information. Find executives and the latest company news.
Read more

ReaMetrix Salary | Glassdoor

No salaries available for ReaMetrix, but Glassdoor has salaries for similar job titles, locations or employers.
Read more

ReaMetrix Interview Questions | Glassdoor

ReaMetrix Logo. ReaMetrix . Is this your company? Overview Overview. 2 Reviews--Salaries--Inter­views--Benefits--Jobs. More. Photos; Follow Add an Interview.
Read more

ReaMetrix Corporate Overview - scribd.com

The document is an overview of ReaMetrix Pvt Ind Ltd.
Read more

ReaMetrix, Inc.: Private Company Information - Businessweek

ReaMetrix, Inc. provides research and development, GMP-level assay development, validation, and testing services for the pharmaceutical ...
Read more

ReaMetrix | AngelList

ReaMetrix - View company info, team members, fundraising and more. Join Log In. AngelList. Startup Jobs; ... Overview ReaMetrix Share There was a ...
Read more

ReaMetrix | crunchbase

ReaMetrix offers R&D, assay development, validation, and testing services for the pharmaceutical, biotechnology, and diagnostic industries.
Read more