Monday lab ppt presentation

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Published on October 10, 2007

Author: Janelle

Source: authorstream.com

Slide1:  SAPS Biotechnology Scotland Project Laboratory Sessions Biotechnology Summer School 2002 Slide2:  Aim of practical sessions To provide hands-on experience of a range of experimental work which: enlivens teaching of molecular biology, biotechnology, genetics & plant science is appropriate for 5-14 through to AH supports curriculum aids pupil understanding Slide3:  Practical Sessions Slide4:  Fruit Full of DNA - Curriculum Simple extraction of DNA from kiwi & strawberry Slide5:  Simple extraction of DNA from kiwi & strawberry Fruit Full of DNA - Curriculum Slide6:  Fruit Full of DNA - method Keep materials cool where indicated Squash fruit in a self-seal plastic bag to mechanically break up cells Add washing up liquid, salt and heat to 60C for 15 mins to disrupt cellular membranes Filter to remove cell debris Precipitate DNA by adding cold methanol Slide7:  Practical session (a) Prepare gel for Protein Electrophoresis: Page 13, ‘Preparing the Gel’; Instructions Nos 1, 4 - 6 Squash fruit in a self-seal plastic bag to mechanically break up cells add washing up liquid, salt and heat to 60C for 15 mins to disrupt cellular membranes Slide8:  Protein Power Slide9:  Protein Power Gel Electrophoresis negative charge conferred on molecules gel is porous molecules travel through gel according to size when electrical current applied smaller travel faster Slide10:  Protein Power Protein within subject specifications Nutrition – demonstrate different foods contain proteins Structure and function – demonstrate proteins of different sizes Slide11:  Protein Power - Curriculum Slide12:  Protein Power - Curriculum Slide13:  Protein Power - Curriculum Slide14:  Protein Power - Method Extract the protein Cut sample – !SIZE MATTERS! – small is beautiful Mash sample Add blue marker dye (Laemmli buffer) – it contains eBlue dyef eGlycerolf eDetergentf Mix by flicking tube Denature proteins by placing in boiling water bath Cool – leave on ice Slide15:  Protein Power - Method Load the gel Gel – pure agarose (3%) Cover with TB Buffer (conducts electricity) Gently remove comb from gel Load wells – clean tip for each sample Work close to electrophoresis tank Slide16:  Protein Power - Method Place in large electrophoresis tank Run the gel Fit one electrode at each end of the tank Slide17:  Practical session (b) Slide18:  Electrophoresis of mixtures of food dyes Wizard Genes Slide19:  Electrophoresis of mixtures of food dyes Wizard Genes - curriculum Slide20:  Electrophoresis of mixtures of food dyes Wizard Genes - curriculum Slide21:  Wizard genes - method Prepare agar gel & allow to set Load wells with ‘chromosome extract’ Insert electrodes and attach crocodile clips Wire electrophoresis tank to batteries Run for 30 minutes Pour off water and examine bands on gel Identify powers possessed by each wizard Slide22:  Practical session (c) Slide23:  Protein Power - Method Staining the proteins Discard electrodes and pour away buffer Wash with deionised water (soak for 1 hour) Shake stain bottle well and pour 12cm3 over gel Leave for 24 hours and wash Results best seen 24 – 48 hours after staining Slide24:  See you tonight for visit to Mary King’s Close Biotechnology Summer School 2002

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