Published on February 17, 2014
is in nalys lite a es of satel nom icro le ge M anel org ta hy rop hlo C
Simple Sequence Repeats (SSRS) Simple Sequence Length Polymorphisms (SSLPS)
Highly Abundant In Prokaryotes And Eukaryotes
Both In Coding And Non-‐Coding Regions
Inherently Unstable Mendelian Manner
Powerful GeneMc Markers： Locus-‐speciﬁc Co-‐dominant PCR-‐based Highly Polymorphic
DistribuMon Of SSRs In The Genome Is Not Random!
Previous: Coding Or Gene-‐rich Regions + Nuclear-‐ Genome Based
Current : DeterminaMon At The Whole Genome Level!
Chloroplast And Mitochondrial Genomes Of Chlorophyta
Organelle genome sequence source
IdenMﬁcaMon of SSRs
1 Frequency Of SSRs In Chloroplast And Mitochondrial Genomes
The Frequencies Of SSRs Chloroplast Mitochondria
Algal Species : No RelaMonship Between The Frequencies Of Simple And Compound SSRs
2 DistribuMon of SSRs in genic and intergenic regions
The Major ProporMon Of SSRs Intergenic Regions Genic And Intragenic Regions
“The diﬀerences in frequency suggest that the polymorphism associated with coding regions is lower than non-‐coding region.” non-‐coding region coding region
“The microsatellite pa^erns making it impossible to measure associated relatedness and polymorphism present within alleles.”
3 SSR Pa^erns
mono-‐nucleoMde repeats, poly A/T.
DinucleoMde repeat pa^erns were less frequent Maximum dinucleoMde repeats was poly AT/TA
The major di-‐ nucleoMde repeat pa^ern in Chlorophyta organellar genomes had shown the similarity with organelle and nuclear genomes of higher taxa
In very minute amounts other repeat pa^ern tetra-‐, penta-‐, hexa-‐ were observed
SSRs in nuclear ESTs of other plant species and some cereal species tri-‐nucleoMde repeats were the most abundant class followed by di-‐nucleoMde repeats
FuncMonal categorizaMon of genes having microsatellites 4
In all chloroplast genomes… In mitochondrial genomes…
The study is important in term of revealing the simple repeat pa^erns in organellar genomes of lower plant species and to measure their abundance and polymorphism.
Suggest the evoluMon of chloroplast and mitochondrial genomes independent of nuclear genomes.
EvoluMonary Factors With Diﬀerent Rate And Randomness: Signiﬁcant Diﬀerences In SSR Pa^erns Among Members Of Same Family
T u! yo ank h
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Microsatellite analysis in organelle genomes of Chlorophyta. (PMCID:PMC3321234) Full Text Citations ; BioEntities ; Related Articles ; External ...
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Microsatellite analysis in organelle genomes of Chlorophyta: Divergence and Shannon information in genomes: The isochore patterns of invertebrate genomes:
open access www.bioinformation.net Hypothesis Volume 8(6) Microsatellite analysis in organelle genomes of (2012) Cached. Download Links