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Microbiological assay

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Information about Microbiological assay
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Published on March 13, 2014

Author: jmpatel1983

Source: authorstream.com

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Microbiological Assay of antibiotics Prepared By: Dr.Jaydeep Patel, Lecturer, B.K.Mody Govt. Pharmacy college, Rajkot : Microbiological Assay of antibiotics Prepared By: Dr.Jaydeep Patel, Lecturer, B.K.Mody Govt. Pharmacy college, Rajkot The cylinder plate method (Method-A): The cylinder plate method (Method-A) Media Standard preparation and unit activity Buffer solutions Preparation of standard solutions Preparation of sample solution Test organisms Preparation of inoculums Apparatus Temperature control spectrophotometer :  Media Standard preparation and unit activity Buffer solutions Preparation of standard solutions Preparation of sample solution Test organisms Preparation of inoculums Apparatus Temperature control spectrophotometer PowerPoint Presentation: 1. One level 2.Two level A. Cup-Plate Method-One level: A. Cup-Plate Method-One level Preparation of standard Calibration Curved A. Cup-Plate Method-Two level: A. Cup-Plate Method-Two level % potency = Antilog (2.0 + a log I) Where, a = (U1+U2)-(S1+S2) ------------------------- (U1+U2)-(S1-S2) I= ratio of dilution potential % potency is between 60% to 150 % of standard The turbidimetric method (Method-B): The turbidimetric method (Method-B) B. Turbidity method : B. Turbidity method Preparation of standard Calibration Curved

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