Published on February 11, 2009
Applications of ICx Protein-Polymer Composites: Wound Care email@example.com ICx BioImmobilization™ Vice President, Commercial Business Development www.icxt.com
About ICx Technologies (NASDAQ: ICXT) Advanced sensor and technology development company Core business focus: military, homeland defense, security markets Founded 2003 25 entrepreneurial teams (companies) located in U.S., UK, Germany 800+ multi-disciplinary workforce. 200+ engineers, 50+ scientists Broad & diversified technology base: 35+ patents , 50+ filed Substantial government development funding 2008 Revenues: $175M Technologies developed within ICx have application outside of security: Wound care, in vitro diagnostics, medical devices We are actively engaged in seeking partnerships to commercialize products utilizing ICx technology and know-how Page 2
ICx-Agentase Agentase operates as a wholly-owned subsidiary of ICx (Pittsburgh, PA) Developing low-cost tools for the detection/decontamination of hazardous chemicals Technical expertise / IP Applied enzymology Formulation development Enzyme-polymer synthesis Urethanes – Fabrics – Gels – Films – Foams Acrylates Water-soluble protein-polymer conjugates – PEG-ylation – PEG-alternatives Scaled synthesis – Bench-top – Pilot – Production Rapid prototyping Launched commercial products based on our technology platform Page 3
ICx BioImmobilization™ ICx BioImmobilization™ Technology Proprietary immobilization of small molecules, enzymes and other proteins into a variety of polymers, covalently bonded within a growing polymer chain Biomolecules act as co-monomers in polymerization synthesis Applications In Vitro Diagnostics Wound healing: ‘smart’ bandages indicate healing progression, or can respond and deliver drugs in situ Medical device coatings: protein-polymer combinations can be pro- healing or can inhibit (anti-microbial, anti-thrombosis, anti-adhesion) Proteins can be immobilized within polymer foams and gels directly (eg., spinal implants) Versatility Polymers can be made into sprays, gels, or foams Pre-polymer material approved by FDA FDA approved and in use as hydrophilic dressings Page 4
ICx BioImmobilization™ Performance Loading of proteins, enzymes or other molecules into polymer allows target analytes to be detected at high sensitivity; Activity and specificity of biomolecules are well maintained for long periods through tertiary structure stabilization; Protein environmental and thermal sensitivity are dramatically reduced; Dyes can be simultaneously loaded into polymers to provide colorimetric indication of analyte presence (eg., wound progress indication); Covalent incorporation ensures molecules will not leach from polymers during use. Experience More than 100 proteins successfully immobilized; Multiple commercial products launched. Page 5
Applying BioImmobilization™ in healthcare Applications In Vitro Diagnostics Development of an analyte-specific sensor for point-of-care IVD Partners interested in working with us because of technology attributes Siemens RapidPoint TM – Stabilization of reagents and proteins » Shelflife: enzymes and reagents formulated to be stable for years without any special storage requirements » Operational: sensors perform reproducibly for long periods of time » Robust: work well under adverse and variable conditions Wound Care Sensors – Identification – Qualification – Quantification Responsive, functional “smart” sponges, gels, materials, coatings, dressings Page 6
BioImmobilization™ for Improved Wound Outcomes: advanced ‘smart’ wound dressings Responsive Delivery of Actives Indicator Sensors Remodeling •Cell number decreases •Collagen fiber bundle organization Proliferative Wound •Fibroblast number increases •Myofibroblasts appear •Connective tissue deposited •Epithelial cell migration Lag •Vascularization (angiogenesis) •Hemostasis •Inflammatory infiltration •Cytokine release Aiba-Kojima, E, et al, Characterization of wound drainage fluids as a source of soluble factors Time associated with wound healing: comparison with platelet-rich plasma and potential use in cell culture, Wound Rep Reg (2007) 15 511–520. Iocono JA, et al, The biology of healing. Oxford University Press, 1998., p.11. Page 7
BioImmobilization™ for the development of ‘Smart’ Wound Care Dressings Potential to synthesize materials that respond to the physiological status of a healing wound Analytical dressings Sensor technology integrated within materials having physical properties of conventional hydrophilic foam dressings Bandage changes color in presence of target analytes in wound – Marker for wound progress / lack of progress – Indicator of common problems or high bacterial colony counts within a wound Self-decontaminating dressings Utilize “decontaminating” enzymes to respond to detected high colony counts by producing disinfectants Smart erodible dressing materials Wound responsive Potential delivery vehicle for actives – Anti-microbials – Large MW drugs – Protein fragments Actively seeking partnerships to exploit these concepts Page 8
Dressings that illustrate conditions in a healing wound ICx has a long history of developing enzyme-containing polyurethanes for use as highly specific and sensitive chemical sensors The same approach may be used to produce ‘indicator dressings’ (either visual or facilitated) for the assessment of the following conditions within a wound (partial list) pH MMPs Color response to increasing MMP / TIMP balance amounts of analyte Small bacterial Large bacterial Microbial colony counts load load Phospholipase A (activates platelet aggregation in an acute wound) NADPH-oxidase (its absence suggests risk of chronic infection) Lysyl oxidase (catalyzes collagen crosslinking in late stage wound healing) NOx Growth factors and cytokines mediated by kinases HGF, IGFs 1 & 2, IFN-γ, IL-6, VEGF, KGF (FGF-7) b-FGF, EGF, PDGF, TGF-α, TNF-a, IL-1, TGF-β Page 9
Summary ICx has proprietary technologies that can be directly applied to wound care, IVD, and medical device space BioImmobilization™ technology is able to incorporate active biomolecules and small molecules within hydrophilic biocompatible polymer foams, gels, and sprays We can use this technology to make ‘smart’ wound dressings to potentially speed the wound healing process Analytical dressings Smart decontaminating dressings Responsive dressings ICx is actively seeking partnerships in wound care to exploit this know-how Page 10
This presentation describes ICx\' BioImmobilizationTM technology and its ... Wound care, in vitro ... In Vitro Diagnostics Wound healing: ‘smart ...
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