Published on December 9, 2016
1. Histology of Male Reproductive System Dr. Laxman Khanal Asst. Professor (Department of Anatomy) BPKIHS, Nepal To MBBS 2nd year 09-12-2016
2. Q. Crystel of Reinke is the characteristic feature of- a. Cell of Leydig b. Sertoli cell c. Spermatogonia d. spermatozoa Q. Most numerous cell organell in Leydig cell is- a. SER b. RER c. Ribosome d. Lysosome Q. Most of the duct system of male reproductive system is lined by ---------- type of epithelium. a. Simple columner b. pseudostratified ciliated columner c. Simple cuboidal d. stratified columner
3. Anatomical subdivision of male reproductive system Male gonad (Testis) Duct system (efferent ductules, Epididymis, vas deferens, ejaculatory duct and part of urethra) Accessory glands- seminal vesicle, prostate and bulbourethral gland (Cowper’s gland) Penis Function of male reproductive system Production, storage , nourishment and transmission of male gametes (spermatozoa) Production of male hormones
4. Developmental site- post abdominal wall Mature position- cooler scrotum (by descent of testis) Histology of testis No of lobule- 200-300 Each lobule – 1-3 ST Covering of the testis 1. T vaginalis 2. T albuginea 3. T vasculosa
5. Seminiferous tubules Cell inside the tubules- spermatogenic cell and Sertoli cell Cell between the tubules- interstitial cell of Leydig •From periphery to center arrangement of the cells is immature to mature cells •Lumen of the tubule contains most mature series of cell (spermatozoa)
6. Blood testis barrier Space between sertoli cells Basal compartment Luminal compartment
7. Function of Testis Functions of Leydig cell • Senses LH and secrets androgen (testosterone) responsible for male type of sexual characters Function of Sertoli cell •Nourishment of spermatogenic cells •Blood testis barrier •Phagocytosis of residual bodies •Senses FSH and secrets ABP •In fetus secrets MIS (suppresses the female genital tract development) Spermatogenic cells- act as male gametes
8. Castor Semenya – 800 m gold medalist in Rio Olympic 2016. She was Found to have suffered from androgen insensitivity syndrome (AIS). • Genotype: 44+XY • Phenotype: Female • Y chromosome present • Leydig’s cells present • Normal androgen level • No receptor found for androgen • no male sex organ develops • Looks like female
9. •Seminiferous tubules •Straight tubules •Rete testis •Efferent ductules (15-20) •Duct of Epididymis •Vas deferens
10. •The epididymis is a long & extremely convoluted duct lying on the posterior aspect of the testis. •Made up of head (efferent ductules), body & tail (duct of Epididymis). •Amount of smooth muscle increases from head to tail direction. •Lining epithelium- pseudostratified columner epithelium with stereocillia. •Function- storage and maturation of spermatozoa. Epididymis
11. Appreciate the single layer of smooth muscle which will increase as it is followed distally. Lining – pseudostratified columnar with stereocilia
12. No muscle layer Uniform shape of tubules Stereocilia absent Muscle layer present Variable shape of ducts Stereocilia present
13. Vas Deferens • Length-45 cm • Continue from tail of epididymis to the seminal vesicle • Lining epth- pseudostratified columner epithelium with few stereocilia. • Multiple layer of smooth muscle (outer and inner layer of longitudinal muscle with circular layer in between)
14. Lining epithelium Three layer of smooth muscle L c L The epithelial lining and lamina propria are thrown into longitudinal folds, permitting expansion of the duct during ejaculation.
15. •The dilated distal portion of each ductus deferens, known as the ampulla, receives a short duct draining the seminal vesicle, thus forming the short ejaculatory duct. •The ejaculatory ducts from each side converge to join the urethra as it passes through the prostate gland.
16. Seminal vesicle •Secrets 70% of seminal fluid which is alkaline •Secretion is rich in fructose, vitamin C and prostaglandins •Lining epth- pseudostratified columner •Lumen is highly irregular and recessed giving a honeycombed appearance
17. Honeycomb appearance – lumen of seminal vesicle
18. Prostate gland • Surrounds the bladder neck and the part of the urethra, known as the prostatic urethra. • In the substance of the gland, the urethra merges with the ejaculatory ducts. • Histologically the prostate is compound tubulo-alveolar glands embedded in a fibromuscular stroma. • Secretion consists of 30% of seminal fluid.
19. In the histological section prostate has arrangement of glands in three concentric layers. 1. Inner zone- mucosal gland 2. Central zone- submucosal gland 3. Peripheral zone- main gland • All the secretion finally secreted into prostatic urethra. Peripheral zone = prone for carcinoma (PSA) Inner zone= prone for BPH Inner zone Central zone Peripheral zone
20. •Prostate = glandular tissue + fibromuscular stroma. •Contains prostatic urethra • Glands are line by simple or pseudostratified columner epithelium. • Secretion is rich in glycoprotein. Sometime lumen of gland contains deposition of glycoprotein as corpora amylacea or prostatic concretion.
21. Benign prostate hyperplasia Testosterone Dihydrotestosterone (DHT) 5α reductase
22. • Found in urogenital diaphragm • Secret preseminal mucus secretion • Help to lubricate the penile urethra
23. Penis • The main components of the penis are three cylindrical masses of erectile tissue, plus the urethra, surrounded by skin. • At its distal end, the corpus spongiosum expands to form the glans penis. • Except for the prostatic part of urethra, and most distal part of urethra lining is pseudostratified columner epithlium.
24. • Corpora cavernosa • Corpus spongiosum • Tunica albuginea • Deep fascia of penis • Superficial fascia
25. Sexual stimulation Parasympathetic Stimulation (S2-S4) Increased blood flow. Smooth muscle relaxation. Erection Reflex sympathetic stimulation (L1) Emission Blood flow decreases. Smooth muscle contracts Contraction of BS & IC Ejaculation