Genetic Technology

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Information about Genetic Technology
Education

Published on January 12, 2009

Author: kdr921

Source: authorstream.com

Genetic Technology : Genetic Technology By: Mr. B. del Rosario 3rd Quarter BIOLOGY Paref-Southridge School What is genetic technology? : What is genetic technology? Simply put, it is the intent to increase the frequency of desired alleles in a population. Possible benefits… : Possible benefits… 1947 milk cows vs. 1997 milk cows Inbreeding develops pure lines. : Inbreeding develops pure lines. Hybrids are usually bigger and better. : Hybrids are usually bigger and better. Why do a test cross? : Why do a test cross? Genetic Engineering : Genetic Engineering allows increased frequency of a desired allele in a faster, more reliable manner DNA fragments are cut/cleaved, then inserted into a host (same or different species) also referred to as recombinant DNA technology Recombinant DNA : Recombinant DNA made by connecting or recombining DNA fragments from different sources Transgenic Organisms : Transgenic Organisms host organism uses the foreign DNA as if it were its own Tobacco plant with a gene from a firefly Recombinant DNA Technology : Recombinant DNA Technology 1st step - isolate the foreign DNA fragment to be inserted. 2nd step – attach DNA to a carrier 3rd step – transfer into the host organism Restriction Enzymes : Restriction Enzymes allows DNA to be cut (a.k.a. DNA-cleaving enzymes); are bacterial proteins Some cut across both strands and produce “blunt ends.” Some cut palindromic sequences that result to the formation of “sticky ends.” Blunt Ends : Blunt Ends EcoRI action : EcoRI action Commonly used vectors : Commonly used vectors Plasmid (of bacteria) Viruses Shotgun technique (blindly shoots tiny particles coated with the gene into the host cells) Micropipette * Vectors may be biological or mechanical. Micropipette : Micropipette Gene gun : Gene gun Gene gun : Gene gun www.agriculture.purdue.edu/.../Genegun.jpg What is a plasmid? : What is a plasmid? Insertion into a vector : Insertion into a vector Gene cloning : Gene cloning Animal cloning : Animal cloning Cloning : Cloning Polymerase Chain Reaction (PCR) : Polymerase Chain Reaction (PCR) Polymerase Chain Reaction (PCR) : Polymerase Chain Reaction (PCR) Polymerase Chain Reaction (PCR) : Polymerase Chain Reaction (PCR) Polymerase Chain Reaction (PCR) : Polymerase Chain Reaction (PCR) References : References images.encarta.msn.com/.../phl/T012584A.gif www.spraguephoto.com/stock/images/El_Salvador... www.beebeesee.tv/images/SkinnyCow.jpg upload.wikimedia.org/wikipedia/commons/4/4a/C... upload.wikimedia.org/.../6/63/Horse-racing-4.jpg www.monteweston.com/Biology/Bio/collflower2.jpg www.bio.davidson.edu/.../Talbert/lucifmed.jpg www.freewebs.com/mohnbon/MouseEar.jpg References : References 9. www.accessexcellence.org/.../SFTS/SFTSg/11.gif 10. www.obgynacademy.com/.../images/chimericDNA.gif 11. www.biotech.ucdavis.edu/.../micropipette.png 12. www.clpmag.com/.../2007-06/2007-06_06-02.jpg 13. fig.cox.miami.edu/.../150/gene/38x15dnagun.jpg 14. science.marshall.edu/.../images/gene%20gun.jpg 15. www.artsci.wustl.edu/~anthro/blurb/fg8.t.gif 16. employees.csbsju.edu/.../ch331/dna/plasmid.gif References : References 17. www.sciencecases.org/dog_cloning/cloning.gif 18. www.lifenews.com/picturecloning2.jpg 19. library.thinkquest.org/.../polystep1.gif

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