Gene Therapy for Ligament and Tendon

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Information about Gene Therapy for Ligament and Tendon
Education

Published on June 14, 2019

Author: tehreemanis

Source: authorstream.com

Gene Therapy for Musculoskeletal Disorders: Gene Therapy for Musculoskeletal Disorders Ligament and Tendon Dr. Tehreem Anis DIRS, DU Introduction: Introduction Ligament Connect two articulating bones across a joint. Maintain joint congruency and guide joint movement Tendon Connect muscle to bone, consist of elastin and collagen fibers. Convert muscle contractions to joint motion. Ligament and Tendon Growth Factors: Ligament and Tendon Growth Factors Platelet derived growth factor-BB (PDGF-BB ) Increase the mechanical properties of a healing ligament Epidermal growth factor (EGF ) Stimulate fibroblast proliferation Insulin-like growth factor (IGF-I ) Stimulate fibroblast proliferation Transforming growth factor-b (TGF-b ) Stimulant of collagen and total protein synthesis BMP-12 Induce ligament and tendon formation. Cells for Gene Therapy Strategies Directed Towards Ligament Regeneration: Cells for Gene Therapy Strategies Directed Towards Ligament Regeneration Adenoviral transfection Transfection efficiency was 90-100% and was detected up to 7 days post-infection. Retroviral transfection Lower at 10-60% Transfection followed by antibiotic selection resulted in a transfection efficiency over90% Transfect actively dividing cells – most appropriate for an ex vivo approach In Vivo Gene Therapy Strategies to Intact Ligament and Tendon: In Vivo Gene Therapy Strategies to Intact Ligament and Tendon Studies indicated a functional exogenous gene may be directly delivered to and continually expressed at ligament and tendon tissue. In vivo studies indicated greater staining in the tissue sheath than the ligament or tendon tissue. Extended expression was also attributed to ligament and tendon tissues being less immunoactive . The virus dose-transfection rate study indicated the transfection rate is directly linked to the quantity of viral particles injected Ex Vivo Gene Therapy Strategies to Intact Ligament and Tendon : Ex Vivo Gene Therapy Strategies to Intact Ligament and Tendon Ex vivo gene transfer results indicated transfected cells were not only found in the synovial layer but also throughout the entire tendon aligned parallel to the pattern of the collagen fibers. Significantly more transfected cells were found using the ex vivo approach Ex vivo approach, transfected cells were able to integrate in the tendon tissue, while the direct injection of adenovirus particles in vivo limited transfection to the tissue sheath and surface tissue layers Ex vivo approach, transfected cells were able to integrate in the tendon tissue, while the direct injection of adenovirus particles in vivo limited transfection to the tissue sheath and surface tissue layers Gene Therapy Strategies for Lacerated Tendon Repair: Gene Therapy Strategies for Lacerated Tendon Repai r The in vivo and ex vivo gene therapies applied to intact ligament and tendon tissues were expanded by determining the ability of these gene transfer strategies to heal lacerated tendon models. Gene Therapy Strategies to Promote Osseo-Integration of Tendon Grafts: Gene Therapy Strategies to Promote Osseo-Integration of Tendon Grafts Gene therapy may also be used at the tendon-bone insertion site to promote osseointegration . Clinical Trials for Ligament and Tendon Replacement: Clinical Trials for Ligament and Tendon Replacement No gene therapy based clinical trials have been reported for the treatment of defects to the ligament or tendon .

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