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Ethanol from Xylose

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Information about Ethanol from Xylose
Education

Published on March 12, 2009

Author: mallikab

Source: slideshare.net

Description

Seminar given to grad student @ Dept of Biotechnology, KKU in Jan 2008.
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Lignocellulose Lignin Glucose Xylose Other sugars Acid & enzyme hydrolysis Strain selection Co-cultivation in glucose/xylose medium Ethanol from xylose : free cells Ethanol from xylose : immobilised cells

Lignocellulose

Saccharomyces cerevisiae Zymomonas mobilis

Saccharomyces cerevisiae

Zymomonas mobilis

Kluyveromyces marxianus Candida shehatae Candida shehatae Ethanol Production phase

Kluyveromyces marxianus

Candida shehatae

Z. mobilis and C. shehatae in 2-stage cultivation

Z. mobilis and C. shehatae in 2-stage cultivation

C. shehatae in fermenter at various pO 2 levels (xylose concentration) 25% 25%

C. shehatae in fermenter at various pO 2 levels (xylose concentration)

C. shehatae in fermenter at various pO 2 levels (biomass concentration)

C. shehatae in fermenter at various pO 2 levels (biomass concentration)

C. shehatae in fermenter at various pO 2 levels (ethanol concentration)

C. shehatae in fermenter at various pO 2 levels (ethanol concentration)

Ongoing experimental works Use C. shehatae biomass immobilised in Ca-alginate beads Varying cell concentration in beads Varying alginate concentration Repetitive cultivation

Ongoing experimental works

Use C. shehatae biomass immobilised in Ca-alginate beads

Varying cell concentration in beads

Varying alginate concentration

Repetitive cultivation

Acid Hydrolysis Enzymatic hydrolysis NH 4 OH Dilute acids (HCl, H 2 SO 4 ) Conc acids (HCl, H 2 SO 4 ) Cellulose, cellobiase Lignin removal Hemicellulose hydrolysis Cellulose hydrolysis

Acid Hydrolysis

Enzymatic hydrolysis

Collaboration with Dr. Atcha Boonmee, Microbiology, KKU Cloning of genes responsible for xylose utilisation C. shehatae as gene donor S. cerevisiae as host cells

Collaboration with

Dr. Atcha Boonmee, Microbiology, KKU

Cloning of genes responsible for xylose utilisation

C. shehatae as gene donor

S. cerevisiae as host cells

Lactic acid production process improvement Integration of external modules for lactic acid removal : improvement towards continuous production Downstream process : recovery without solid waste (collaboration with Dr. Arthit Neramittagapong, Chem. En., KKU) Xylose utilisation for production of chemicals

Lactic acid production process improvement

Integration of external modules for lactic acid removal : improvement towards continuous production

Downstream process : recovery without solid waste (collaboration with Dr. Arthit Neramittagapong, Chem. En., KKU)

Xylose utilisation for production of chemicals

 

University library

University library

Walkway between Electrical Engineering and Mechanical Engineering

Walkway between Electrical Engineering and Mechanical Engineering

Overlook from middle campus

Overlook from middle campus

Biological Sciences Building

Biological Sciences Building

One of lab bench in my G17 lab

One of lab bench in my G17 lab

In G17 with Martin, my lab manager

In G17 with Martin, my lab manager

In G17 lab with Nick

In G17 lab with Nick

Also in G17 lab with Caine and Tony

Also in G17 lab with Caine and Tony

Pilot plant as part of my lab

Pilot plant as part of my lab

Another view of the lab

Another view of the lab

Trichoderma harzianum cultivation in 5-L fermenter

Trichoderma harzianum cultivation in 5-L fermenter

Normal set-up of batch fermentation system

Normal set-up of batch fermentation system

Set-up for ion exchange resin regeneration loop

Set-up for ion exchange resin regeneration loop

Set-up of fermentation system with electrodialysis (1)

Set-up of fermentation system with electrodialysis (1)

Set-up of fermentation system with electrodialysis (2) Electrodialysis system

Set-up of fermentation system with electrodialysis (2)

Electrodialysis system

 

May you all cross the bridge Over all the obstacles Towards the success you wish … HAPPY NEW YEAR 2008 …

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