Embryo Biopsy - The Finer Points of Trophectoderm Biopsy - Suzanne Cawood CRGH ESHRE 2012 - RI presentation

33 %
67 %
Information about Embryo Biopsy - The Finer Points of Trophectoderm Biopsy - Suzanne...
Health & Medicine

Published on December 20, 2013

Author: RI_UK

Source: slideshare.net


The Finer points of Trophectoderm Biopsy Embryology, Suzanne Cawood Embryologist at CRGH - with lead Alpesh Doshi

The finer points of trophectoderm biopsy Suzanne Cawood The Centre of Reproductive and Genetic Health, London. UK

Preparing for blastocyst biopsy  Excellent blastocyst culture system - KPIs (Blastocyst formation rate, utilisation rate, %d5 vs. d6 formation rate  Group/individual culture until d5. If group culture, separate blasts morning of d5/6 into individual culture  Zona drilling d3 – ensure hole size optimum (too large, risk of premature cell loss; too small, risk of ‘pinching’ of trophectoderm cells) Biopsy in HEPES media. Make dishes 1 hour prior to procedure warmed at 37°C (non-gassed)  Ensure microscope stage heated to temperature which ensures droplet temperature is at 37 °C  Ensure optimum service is provided. Biopsy at d5AM/PM + d6 AM/PM is possible.

When and what to biopsy? WHEN?  Take into account how long the diagnosis will take.  Vitryfying blastocysts post biopsy? Or aiming for fresh embryo transfer? WHAT?  Hatching or Hatched blastocysts. Hatching more ideal  If herniating – trophectoderm cells will have to be ‘sucked’ from zona – more risk of lysis and damage to the embryo  ICM position

The procedure Rapid technique Minimise trauma/damage to both embryo and cells biopsied Use blastocyst holding pipette Common difficulties: Positioning of embryo/pipettes (Hatched blastocysts, ICM hatching out) Do not take too many cells! Remember image is 3D Control at all times – both holding, suction and laser. Utilise foot pedal if possible. Minimise number of laser shots – maximise shot value. Laser shots between the junction of trophectoderm cells Post biopsy, cells to be washed (PBS-PBA) and tubed (hand-pipetted) into empty eppendorf tubes

Technique of excising cells  Laser shot and tear cells  Fewer laser shots and ‘brushing’ the cells off using the holding pipette. - Difficulty: Cells are sticky! - more likely to leave cells on holding/aspiration pipette (potential contamination so ensure pipettes changed if necessary) - this technique has to be used for completely hatched blastocysts

Add a comment

Related presentations

Related pages

Alpesh Doshi - Finer points of biopsying - ESHRE 2013 ...

This film is part of a presentation "Finer Points of Trophectoderm Biopsy using a Saturn 5™ laser" given by Alpesh Doshi, Head of Embryology ...
Read more

Saturn 5 Active Biopsy Mode - YouTube

Saturn 5 Active Laser Biopsy Mode. ... Finer points of trophectoderm biopsying ... RI's 25 hours of ESHRE 2015 in 140 seconds - Duration: ...
Read more

Preimplantation genetic diagnosis - Wikipedia, the free ...

Preimplantation genetic diagnosis provides a method of ... for trophectoderm cells). The biopsy procedure ... to biopsy embryos at the ...
Read more

ESHRE 2012 Presentacion Simposio Ferring - Documents

Embryo Biopsy - The Finer Points of Trophectoderm Biopsy - Suzanne Cawood CRGH ESHRE 2012 - RI presentation The Finer points of Trophectoderm Biopsy ...
Read more

Research Instruments | Leading edge technology and medical ...

Research Instruments Ltd (RI) ... embryos and biopsied ... PGD Biopsy. RI offers a good range of products to make Preimplantation Genetics Diagnosis ...
Read more

Welcome! [bookalltt.ru]

Read more

Health and Wellness - USATODAY.com - USA TODAY: Latest ...

... Evidence definitively shows Zika causes birth defects The declaration marks a "turning point" in the scientific understanding of the ... USA TODAY Shop;
Read more


OrganizedWisdom. OrganizedWisdom ...
Read more