Egg Windowing

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Information about Egg Windowing
Education

Published on January 11, 2014

Author: CNuggets

Source: slideshare.net

Description

I love eeeeggs! (◠‿◠✿)

June Hayden R. Sinson Ramon Magsaysay High School - Espaǹa Arturo A. Tolentino, Caloocan City Science High School

 The study of development has been greatly aided by the use of the chick embryo as an experimental model.

It is necessary to open the eggshell and reclose it without perturbing the embryo's growth. The embryo can be examined at successive developmental stages by re-opening the eggshell. 

Protocol

Maintain at 37°C with relative humidity that was set above 60%, remove the eggs from the incubator.

 Turn eggs 90 degrees so that the large base lies horizontal where your embryo is located.

Saturate a stack of nonsterile gauze with 70% ethanol. Use two to three pieces to swab up to 5 eggs. Discard when the gauze is soiled.

 Cut and place a 1" x 1" piece of plastic tape just left of the base to protect the area where the albumen will be drawn out.

 Use the point of a pair of scissors to make a small hole in the middle of the tape.

 Using a 10 cc syringe with an 18gauge, 1-inch needle, slowly drill the needle through the hole made by the scissors.

 Drive the needle down at a 45°C angle towards the bottom of the egg.  Tilt the needle towards the center and draw up 3 to 4 mL of albumen.

 Cut a 3" x 3" piece of plastic tape and stretch it to fit on the top of the egg. Extend the corners of the square around the rounded ends of the horizontal surface of the eggs, being careful not to pull too hard. Pull the tape so that it is tight against the surface of the eggs with no folds.

 Using a pair of sharpstraight 4" dissection scissors, twist a hole into the bottom center of the area where the tape was placed.

 Slowly guide the lower blade of the scissors into the egg being sure to keep the tips up against the inside of the shell. Direct the blade towards the base and slowly begin to cut the shell.

 Remove the scissors and repeat going in the opposite direction until only a small bit of the egg remains attached. Check to be sure the egg is fertilized. Shut the window.

 Cut about a 2-3" long by 1/2" wide plastic tape and shut the window so it fits back into the hole that was cut. Take another 1 x 1" piece of tape and seal the hole from which the egg was drained. Use a pair of forceps to reopen the egg to do any manipulations. When you're ready to return the eggs to the incubator, cut a piece of tape that is large enough to seal the window and cover the entire horizontal surface of the egg.

 http://www.ncbi.nlm.nih.gov  J Vis Exp. 2007; (8): 306. Published online 2007 October 1. doi: 10.3791/306 PMCID: PMC2562489 Matthew J. Korn and Karina S. Cramer  http://www.youtube.com : Windowing Chicken Eggs for Developmental Studies

Avian egg is a useful animal model for studies concerning early embryonic development. The model is particularly advantageous of the ease by which the avian embryo may be accessed and handled. Numerous bioassays revolving around avian embryos have been developed..

Avian eggs may be used for teratogenicity studies. Choriooallantoic membrane (CAM assay. Egg windowing • Process by which the egg is opened and the embryo revealed for manipulation.

1.Perform egg candling in order to determine the position of the embryo. This can be done by holding the egg in front of a light source.

(Source:http://www.pic2fly.com/Candling-Chicken-Egg-Pictures.html) This step is necessary in order to ensure that the windowing be performed exactly on the top of the embryo

2. Using sandpaper, scrape the egg shell directly on top of the embryo. 3. Once the shell is thin enough, cut a small window (1 cm x 1 cm) into the egg shell. Remove the egg shell first before cutting through the shell membrane. Once the window has been cut, it may be enlarged depending on the need.

4. Observe the embryo. 5. Add 10 drops of the test reagent. 6. Cover the open window with parafilm and return to the incubator. References: G. Speksnijder & R. Ivarie. 2000. A Modified Method of Shell Windowing for Producing Somatic or Germline Chimeras in Fertilized Chicken Eggs. Poultry of Science 79:1430-1433. UP seminar materials , acquired, April, 2013.

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