Published on February 22, 2014
EFFECT OF THE DIFFERENT AUXINS AND CYTOKININS IN CALLUS INDUCTION, SHOOT, AND ROOT REGENERATION IN SUGARCANE Presented by: Arghya Narendra Dianastya 5620000087 Departement of Agronomy Kasetsart University, Kamphaeng Saen
INTRODUCTION Modern sugarcane varieties are breeding of species, involving crosses between the species Saccharum officinarum L. and S. spontaneum L (Heinze,1987). Sugarcane is a tall growing monocotyledonous crop. Sugarcane has ability to store high concentrations of sucrose, or sugar, in the internodes of the stem.
SUGARCANE PRODUCER In 2010, Brazil was the largest producer at 719 million tonnes (FAO, 2013)
GLOBAL PRODUCTION AND CONSUMPTION FAO’s current estimate for world sugar production in 2012/13, at 180 million tonnes, (new record production)
SUGARCANE AREA HARVESTED
INTRODUCTION What can we do to improve the quality and productivity of sugarcane
IMPROVEMENT OF SUGARCANE PRODUCTIVITY AND QUALITY Plant improvement Conventional methode Plant biotechnology (ex: plant tissue culture) Cultural practice improvement
ADVANTAGES OF TISSUE CULTURE Plant regeneration through tissue culture technique would be a better alternative for improving the quality, production, and improvement of crop through the rapid production of clones (Jabbot & Atkin, 1987)
WHAT IS TISSUE CULTURE ? Plant tissue culture can be defined as a procedure used to mantain and grow plant tissue (callus, cells, protoplasm) and organ (stem, root, embryos) in aseptic or in vitro culture. Basic Concept is Totipotency : Totipotency ability in individual plant cells that can be regenerated to a whole plant by modulating culture conditions (Reinert, 1959).
PATHWAY OF PLANT TISSUE AND CELL CULTURE (Hartmann et.al., 1990 )
OBJECTIVES OF THE RESEARCH To analyze the effect of different concentration of auxins and cytokinins in Callus Induction, Shoot, and Root Regeneration in Sugarcane. To analyze the effect of different level of pH and sucrose in root regenenation of sugarcane.
MATERIAL Sugarcane Cultivar CO 671 (3-5 months old) MS Medium - Murashige and skoog medium Cytokinin BAP Kn Auxin IBA IAA NAA
CALLUS INDUCTION Effect of different concentration, auxin and 2,4-D on callus induction from leaf sheath explants of sugarcane
CALLUS INDUCTION The best callus induction suplement 3.0mg/L of 2,4-D = 85% explant produced callus No callus induction IBA
SHOOT REGENERATION Effect of the cytokinin (BAP,Kn) and the auxin (IBA,NAA) at different concentrations and combination is Ms medium on shoot on shoot regeneration from the callus tissue of sugarcane
SHOOT REGENERATION cont.. Effect of the cytokinin (BAP,Kn) and the auxin (IBA,NAA) at different concentrations and combination is Ms medium on shoot on shoot regeneration from the callus tissue of sugarcane
SHOOT REGENERATION BAP+IBA The best respond 1.0+0.5 mg/L = 95 % produced shoot BAP+NAA The best respond 1.0+0.5 mg/L = 94% produced shoot
ROOT REGENERATION Effect of different auxin on formation of root on the invitro grown micro shoots Cultured
ROOT REGENERATION The Best Rooting Media ½ strength of MS medium + 3mg/L NAA = 95% explant produced root Comparison Media Used NAA and IBA were better response than IAA for producing roots.
CONCENTRATION OF SUCROSE Effect of sucrose concentration on rooting of invitro differentiated shoots of sugarcane
CONCENTRATION OF SUCROSE The Best Sucrose Concentration 50 gram/L of sucrose = 96% explant produced root
pH LEVEL Effect of different pH level on rooting of in vitro differentiated Shoots of sugarcane
pH LEVEL The Best pH Level pH 5.7 = 96% explant produced root The Poorest pH Level pH 3 = 0 % explant produced root
CONCLUSION The best callus induction was observed at 3.0 mg/L of 2,4-D The best shoot regeneration was observed at 1.0 mg/L and 0.5 mg/L of (BAP + NAA) The best root regeneraration was observed at 3.0 mg/L of NAA The best sucrose concentration 50 gram/L of Sucrose The best pH level pH 5.7
REFERENCE Gopitha A., Lakshmi B., Senthilmanickam J. 2010. Effect of the different auxins and cytokinins in callus induction, shoot, root regeneration in sugarcane. International Journal of Pharma and Bio Science. Vol.1/Issue-3/JulSep.2010.
Reinert J (1959) The control of morphogenesis and induction of adventitious embryos in cell cultures of carrots. Planta 53:318–333. Jabbott A.J and Atkin R.A. 1987 Improving Vegetatively Propagated Crop. Academic Press Limited. Hartmann H.T., Kester D.E., Davies F.T., and Geneve R.L. 1997. Plant Propagation: Principles and Practices, Sixth Edition. Prentice Hall International, Inc.
ACKNOWLEDGMENTS Kasetsart University Advisor Prof. Sontichai Chanprame, Ph.D Prof. Sermsiri Chanprame, Ph.D Jember University Advisor Dr. Kacung Haryono CAB (Center of Agriculture Biotechnology) And for All that have helped me to study in Kasetsart University
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