diagnosis

60 %
40 %
Information about diagnosis
Education

Published on February 2, 2009

Author: shabanko

Source: authorstream.com

LABORATORY DIAGNOSIS OF PARASITIC INFECTIONS : LABORATORY DIAGNOSIS OF PARASITIC INFECTIONS By: Dr.Maha jaafar Case diagnosis : Case diagnosis History (Age, occupation, residency, previous infection) Complaint Clinical examination Invesigations - Laboratory investigations - Radiology - Surgical intervention (Exploratory) Provisional diagnosis Confirm the diagnosis Slide 4: Acetic acid RBC haemolysis Clear ova Ether Dissolve fat M.f URINE EXAMINATION : URINE EXAMINATION SEDIMENTATION CONCENTRATION 15-20 min Centrifuge (2 min) Clean conical glass receptacle URINE EXAMINATIONMembrane filtration technique : URINE EXAMINATIONMembrane filtration technique air 10 ml urine Nucleopore filter Eggs of Schistosoma + Saline URINE EXAMINATION : URINE EXAMINATION Slide 10: STOOL EXAMINATION STOOL EXAMINATIONTemporary : STOOL EXAMINATIONTemporary Saline smear Iodine smear saline Iodine 1% Huge number of: Eggs Protozoal troph. Motility (Amoeb, flagellates) Huge number of: Cyst morphological details STOOL EXAMINATIONScanty infectionConcentration techniques : STOOL EXAMINATIONScanty infectionConcentration techniques Sedimentation Floatation Heavy eggs (Ascaris egg) Operculated eggs (Trematodes) Larvae (Strong sterc.) Cysts Non Operculated eggs Trematodes ( S. m.) Cestode Nematode(Hookworms,Trichostong) Cysts STOOL EXAMINATIONSaline sedimentation : STOOL EXAMINATIONSaline sedimentation 10 g stool Saline Mesh wire gauze Conical flask Sediment Emulsify STOOL EXAMINATION Formol Ether Sed. Conc. : STOOL EXAMINATION Formol Ether Sed. Conc. 10% Formalin 1 g stool Sediment formalin debris Ether Thorough mixing Ether Ether adsorbs fecal debris & floats. Formalin fixes & preserves the specimen. Conical flask centrif. tube STOOL EXAMINATION : STOOL EXAMINATION Tin container 20 min Centrif. 2 min Seive Clean light eggs & cysts STOOL EXAMINATIONPermanent Stained smears : STOOL EXAMINATIONPermanent Stained smears Iron haematoxylin stain Trichrome stain Modified Ziehl Neelsen satin (Crpto.) STOOL EXAMINATION Kato technique : STOOL EXAMINATION Kato technique Mesh screen Template Hole Remove the template Cellophane soaked by glycerin (clears faeces( Egg count/ g stool Egg quant. Of: Ascaris, T. trich., Hookworms, S. mansoni STOOL EXAMINATION Stoll’s technique : STOOL EXAMINATION Stoll’s technique NaOH 4 g Stool Erlynmeyer flask 56 CC 60 CC Shake well 0.15 CC Egg count/ slide Eggs/1g= Eggs/slideX100 Egg/day=Eggs/1g X stool wt/g in 24 hrs 24 hr stool Egg quant. Of: Ascaris, T. trich., Hookworms, S. mansoni STOOL EXAMINATION Baermann’s technique : STOOL EXAMINATION Baermann’s technique Warm water Stool/soil seive Glass funnel clamp 30 min 25-50 CC centrifuge Detec. Of Nematode L. /stool, soil STOOL EXAMINATION Cultures for Nematode larvae : STOOL EXAMINATION Cultures for Nematode larvae Filter paper culture Scanty infection Larvae of: St. stercoralis (A,L) Hookworms Trichostrong Water Sealed petri dish Filter paper Slide Slide 27: NaOH Sputum Centfifuge Slide 30: floor Edge BLOOD EXAMINATIONBLOOD FILMS : BLOOD EXAMINATIONBLOOD FILMS Thin Thick Bld drop spread Air dry methyl alcohol Geimsa Air dry Geimsa Circular motion Malaria, Babesia, Filaria, Tryp. BLOOD EXAMINATIONBuffy coat film : BLOOD EXAMINATIONBuffy coat film centrifuge RBC WBC (BC) plasma Citrated bld 30 min Air dry Fix spread Geimsa Tryp., L. donovani BLOOD EXAMINATIONQBC technique : BLOOD EXAMINATIONQBC technique centrifuge RBC RBC +parasite Microhaematocrit tube Acridine orange Malaria, Filaria, Trypanosomes BLOOD EXAMINATIONKNOTT’S CONC. TECHNIQUE : BLOOD EXAMINATIONKNOTT’S CONC. TECHNIQUE 10 ml 1 ml Air dry fix Geimsa Citrated bld Formalin 2 % sediment 2 min centrifuge Filaria INDIRECT IMMUNOLOGICAL METHODS : INDIRECT IMMUNOLOGICAL METHODS Scanty infection. Tissue parasite no portal of exit (Hydatid dis.) Migratory stage (Fasciola) Chronic infection fibrosis (Bilharziasis) INDIRECT IMMUNOLOGICAL METHODS : INDIRECT IMMUNOLOGICAL METHODS IHAT LAT + Sensitized Sheep’s RBC (O–ve) Ag Patient’s serum (?? AB) Agglutination + Agglutination Ag Latex particle Patient’s serum (?? AB) INDIRECT IMMUNOLOGICAL METHODSINDIRECT FLUORESCENT ANTIBODY TEST : INDIRECT IMMUNOLOGICAL METHODSINDIRECT FLUORESCENT ANTIBODY TEST parasite Patient’s serum (?? AB) Anti human AB fluorescein INDIRECT IMMUNOLOGICAL METHODSELISA : INDIRECT IMMUNOLOGICAL METHODSELISA OPD OPD Flat bottom plastic micrititre plate Ag Patient’s serum (?? AB) Anti human AB Peroxidase E AB INDIRECT IMMUNOLOGICAL METHODSCFT : INDIRECT IMMUNOLOGICAL METHODSCFT Ag Patient’s serum (?? AB) complement Anti sheep AB Sheep’s RBC -ve Ab +ve Ab haemolysis No Sheep RBChaemolysis Tube / microplate AB INDIRECT IMMUNOLOGICAL METHODSDouble Electro Immuno Diffusion : INDIRECT IMMUNOLOGICAL METHODSDouble Electro Immuno Diffusion +ve -ve Ag Ab Buffered gel Electric current Line of ppt INDIRECT IMMUNOLOGICAL METHODSImmunodiagnostic Strip Test (Dip Stick Test) Ag : INDIRECT IMMUNOLOGICAL METHODSImmunodiagnostic Strip Test (Dip Stick Test) Ag Nitrocellulose strip Monoclonal Ab Coloured dye Pt bld (?Ag) +ve -ve Malaria, Filaria, African tryp. MOLECULAR BIOLOGICAL TECHNIQUESDNA Probes : MOLECULAR BIOLOGICAL TECHNIQUESDNA Probes DNA Probe Commercially prepared DNA sequence Radio active material Nitrocellulose paper Sample (Serum/ stool) ?? parasite +ve parasite Hybridization Radioactivity MOLECULAR BIOLOGICAL TECHNIQUES Polymerase Chain Reaction (PCR) : MOLECULAR BIOLOGICAL TECHNIQUES Polymerase Chain Reaction (PCR) Single stranded DNA Replication Detection T cruzi, T gondii

Add a comment

Related presentations

Related pages

Diagnosis - Wikipedia, the free encyclopedia

Diagnosis is the identification of the nature and cause of a certain phenomenon. Diagnosis is used in many different disciplines with variations in the use ...
Read more

DIAGNOSIS | Kulturwissenschaftliche Dienstleistungen

Guten Tag! Hier finden Sie kulturwissenschaftliche Dienstleistungen – demnächst ganz neu! Denn diese Seite wird gerade umgebaut. Herzlichen Dank für ...
Read more

dict.cc Wörterbuch :: diagnosis :: Deutsch-Englisch ...

Dieses Deutsch-Englisch-Wörterbuch basiert auf der Idee der freien Weitergabe von Wissen. Mehr informationen! Enthält Übersetzungen von der TU Chemnitz ...
Read more

Medical diagnosis - Wikipedia, the free encyclopedia

Medical diagnosis (abbreviated Dx or D S) is the process of determining which disease or condition explains a person's symptoms and signs. It is most often ...
Read more

Diagnosis | Define Diagnosis at Dictionary.com

Diagnosis definition, the process of determining by examination the nature and circumstances of a diseased condition. See more.
Read more

Diagnosis | Definition of Diagnosis by Merriam-Webster

The unusual combination of symptoms made accurate diagnosis difficult. She is an expert in the diagnosis and treatment of eye diseases. The diagnosis was a ...
Read more

Symptom Checker from WebMD. Check Your Medical Symptoms.

The WebMD Symptom Checker is designed to help you understand what your medical symptoms could mean, and provide you with the trusted information you need ...
Read more

Diagnosis

Diagnosis.com provides comprehensive health and medical information and symptoms and diagnosis information about hundreds of disease and conditions.
Read more

Diagnosis | definition of diagnosis by Medical dictionary

diagnosis [di″ag-no´sis] 1. determination of the nature of a cause of a disease. 2. a concise technical description of the cause, nature, or ...
Read more