chitosan based scaffold for tissue enginnering

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Information about chitosan based scaffold for tissue enginnering
Technology

Published on April 27, 2014

Author: arunbiotec

Source: slideshare.net

Description

Chitosan based bio-polymer play important role in the construction of tissue engineering scaffold.

Tissue Engineering of Nasal Septum Using Chitosan- Based Material by Arunkumar rengaraj (22141800)

Introduction • Nasal septum divides the nasal chamber into two cavities. • Conventional repair of nasal septum involves autologous rib or septum cartilage grafts and prosthetic devices. • Its having limitations like tissue availability, and prosthesis related infection and extrusion. • The objective of my work is to use chitosan based scaffold for nasal septum tissue engineering. • Chitosan, a linear polysaccharide derived from crustacean shells and from many natural sources. • It having advantages like polycationic nature, biodegradability, biocompatibility, mucoadhesiveness. • In addition, it enhances epithelial permeability through the opening of tight junctions between epithelial cells.

Materials and methods 2 % solution of chitosan chitosan + 1 M acetic acid chitosan-collagen membrane Tissues (Human inferior turbinates ) were treated with 0.5% pronase+1:1 mixture (DMEM) + Ham’s nutrient F12 centrifugation suspended in DMEM/F12 1.5-mL cell suspension was seeded on collagen coated Morphological Examination • The ciliary beat was observed under inverted microscope (Leica, DMI 6000, Solms, Germany) with 400 magnification. • Imaging of cilia movement was recorded at 240 frames per second with a high-speed complementary metal oxide semiconductor (CMOS) camera (CMC1300; VDS Vossku hler GmbH, Osnabru ck, Germany). spread on a glass plate Evaporated at 50C Mix 40 μg type I collagen

Reference • Dong-Won Lee, Shawna A Shirley, Richard F Lockey and Shyam Mohapatra. Thiolated chitosan nanoparticles enhance anti- inflammatory effects of intranasally delivered theophylline. Respiratory Research 2006; 7:112. • Tsung-Wei Huang, Yi-Ho Young, Po-Wen Cheng, Yen-Hui Chan, Tai-Horng Young. Culture of Nasal Epithelial Cells Using Chitosan-Based Membranes. The Laryngoscope 2009; 119:2066-2070.

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