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Candida antarctica-lipase-b-liquid-calb-recombinant-product-information-sheet

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Information about Candida...
Technology

Published on February 19, 2014

Author: fermentabiotech1

Source: slideshare.net

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Ilbl ---LlQUID--- FERMASE CALB™. TECHNICAL DATA SHEET lOL FBL's Biotech Division -Inspired by life. FermaseCALB™10L is a recombinant Candida antarctica lipase B enzyme expressed in yeast cells. Product type Fermase CALB™ 10L is supplied as formulated; enzyme concentrate equilibrated with buffer and permitted preservatives. Application Resolution of chiral alcohols and amines; synthesis oHatty-acid esters; versatile applications in organic synthesis; exhibit good to excellent stereoselectivity. Description Yellowish green to green solution. Colour may vary from batch to batch and the colour intensity is not an indication ofthe product quality. Enzyme activity NLT 10000 TBU/mL (Tributyrin hydrolysis). NLT 4500 PLUlmL ( Propyllaurate synthesis). Unit definition One TBU enzyme unit corresponds to amount of enzyme catalyzing the formation of 7 micromole of butyric acid at 40°C, pH 7.5 at 70 % (vlv) tributyrin concentration per minute per ml enzyme in the milieu of 700 mM sodium phosphate buffer. One PLU enzyme unit corresponds to amount of enzyme catalysing the synthesis of 7 micromole propyllaurate per minuteper g ofimmobilized enzyme at60°Cfrom lauric acidand 7-propanol. Reaction schematics For TBU activity H2C-OH (C4 H70 )-O-CH 2 I I (C4 H70 )-O-CH 2 (C4H70)-O-CH2 + 3H20 I Fermase CALB™10L CH2-COOH + HC-OH I H2C - OH Tributyrin Butyric acid For PLU activity H ° II ~OH Lauric acid 3CH3-CH2 - H H I I I I I I H H H + HO-C-C-C-H l-Propanol Propyllaurate Glycerol

Ilbl - - - LlQUIn--- lOL FERMASE CALB™. Stability profile of Fermase CALB™1 OL pH stability of Fermase CALB™10 L Activity (TBU/mLI 14000 12000 10000 8000 6000 4000 Temperature stability of Fermase CALB™10 L . .". Activity (TBU/mLI 14000 12000 10000 8000 6000 4000 2000 o +---,--.-------r-.------r--.----.-----"I~--, o +---.-----r----r-.--...----.-----r----r-.--...----, o 1 2 3 4 5 6 7 8 9 10 11 o 10 20 30 40 50 60 70 80 90 Temperature ('CI pH Storage stability (at 5°C) of Fermase CALB™ 10L Adivity (TBU/mLI 12000 11000 10000 9000 8000 7000 6000 5000 +-r-.--r-r-TT..-r-.--r-r-TT..-r-.--r-r-TT..-r-, 1 13 30 45 62 79 96 110133167215252 Days • Higher stability Advantages • • Storage& product stability Cool and dry conditions are recommended for storage. The recommended temperature for storing is between SaC to SaC. When stored under these conditions, the product will maintain its declared activity for at least 12 months. Safety Fermase CALB™ 1OL is very safe to handle, as it does not generate any aerosols. Avoid direct contact with skin and eyes. Avoid forming aerosols, wash contaminated clothing and ensure sufficient ventilation. Material support data • • Packing size Minimum pack size: 1 kg Bulk pack size : 2S kg Higher activity Broad application Technical data sheet Material safety data sheet • • Certificate of analysis Protocols for assay methods Manufactured By: Fermenta Biotech Limited 'DIL' Complex, Ghodbunder Road, Majiwada, Thane(West)-40061 0, Maharashtra,lndia. Tel: +91 2267980800/888 • Fax: +91 22 6798 0899 Email: info@fermentabiotech.com

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