aseptic techniques

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Information about aseptic techniques

Published on September 27, 2012

Author: vhbhaskar


Aseptic Technique : Guided by: Naresh Rajgor Presented By: Viren Patel Aseptic Technique M.P. Patel College of Pharmacy, Kapadwanj , Gujarat Aseptic Technique : Aseptic Technique Aseptic technique is the technique for manipulations of compounded sterile products and parenteral preparations that prevents contamination Aseptic technique requires specific manipulations for: Syringes Needles Vials Ampules Removal of packaging Assembling of sterile products Hand placement Aseptic Technique - Syringes: Aseptic Technique - Syringes NEVER TOUCH Tip or Plunger Aseptic Technique - Syringes: Aseptic Technique - Syringes 1.5ml measured Aseptic Technique - Syringes: Aseptic Technique - Syringes Syringes are available in sizes ranging from 0.5 to 60 milliliters (ml) Graduation marks on syringes represent different increments depending on size of syringe DO NOT use syringes whose gradations are greater than twice the volume being measured To maintain sterility, neither the syringe tip or plunger should be touched Aseptic Technique - Needles: Aseptic Technique - Needles Hub Where the needle attaches to the syringe tip and allows the fluid in the syringe barrel Bevel The tip of needle is slanted to a point and the slanted part of the needle is the bevel. The bevel allows for smooth insertions through stoppers and ports with minimal coring Aseptic Technique - Needles: Aseptic Technique - Needles Coring The development of a core or hole in the rubber of a vial To prevent coring insert needle as shown Insert the bevel tip first, then pressing downward and toward the bevel so the bevel tip and heel enter at the same point Aseptic Technique - Needles: Aseptic Technique - Needles Needle size is determined by two numbers Gauge Length The larger the gauge number the finer the diameter of the needle’s bore 27 gauge needle finer than 13 gauge needle Common needle sizes at UK include 16G 1&1/2 inch 18G 1&1/2 inch 20G 1 inch Needle length is measured in inches Never touch any part of the needle Open needle package within hood to maintain sterility Aseptic Technique - Needles and Syringes : Aseptic Technique - Needles and Syringes It is important that when using needles and syringes in product preparation that they not be removed from the hood workspace Needles and syringes must be properly disposed in a sharps container located near the hood to prevent injury to others Never dispose of needles or syringes in regular trash Aseptic Technique - Vials and Ampules: Aseptic Technique - Vials and Ampules Vials Ampules Aseptic Technique - Vials and Ampules: Aseptic Technique - Vials and Ampules To prevent contamination Swab rubber closure with 70% alcohol using firm strokes in the SAME direction To prevent core formation Insert needle to penetrate the rubber closure at same point with both tip and heel of bevel To prevent vacuum formation Inject an equal amount of air for the volume of fluid to be removed Reconstituting drug powder Remove an equal amount of air for the volume of diluent added Aseptic Technique - Vials and Ampules: Aseptic Technique - Vials and Ampules Aseptic Technique - Vials and Ampules: Aseptic Technique - Vials and Ampules To break ampule Clean ampule neck with alcohol swab Leave swab in place Grasp ampule neck with thumb and index finger Use quick, firm, snapping motion away from body towards side wall of hood DO NOT BREAK TOWARD HEPA FILTER Aseptic Technique - Vials and Ampules: Aseptic Technique - Vials and Ampules To withdraw medication from ampule Tilt ampule Place needle bevel in corner space near opening Pull back syringe plunger Do NOT use a filter needle to remove ampule contents To avoid glass contamination of ampule solution Use 5 micron filter needle to filter glass as solution is pushed out of the syringe A filter needle can only be used in one direction, otherwise glass particles originally filtered are reintroduced For UK consistency always use the filter needle as the second needle Aseptic Technique - Vials and Ampules: 1 2 3 4 Aseptic Technique - Vials and Ampules Aseptic Technique - Removing Packaging: Aseptic Technique - Removing Packaging Any sterile component or supply (e.g., syringes, needles, and ampules) should only be opened and/or removed from their packaging within the laminar-flow workspace. When opening the wrapper on a needle, it should be peeled open. Tearing paper introduces paper particles into the hood which could lead to product contamination. Aseptic Technique - Assembling: Aseptic Technique - Assembling Great care must be exercised to prevent any touch contamination of critical edges or component surfaces. The syringe tip and plunger and all parts of the needle are critical surfaces. Aseptic Technique - Hand Placement: Aseptic Technique - Hand Placement It is important that hand placement during any aseptic manipulation is such that laminar airflow is not interrupted around any of the critical sterile fluid pathways. Great care should be taken to avoid contact with the syringe plunger, especially during multiple manipulations using the same syringe. Since the plunger enters the barrel of the syringe during repeated uses, contamination can be accidentally introduced into the barrel. Summary: Summary Aseptic technique is the manipulation of sterile products to prevent contamination Giving a patient a contaminated product can cause serious adverse effects including DEATH Discard any potentially contaminated product and inform the pharmacist that product was discarded STORAGE AND DISTRIBUTION : STORAGE AND DISTRIBUTION Closed System With Air Exchange Through Filter That Remove Micro –Organism, And Vapours From The Air As The Tank Is Filled And Emptied ? They May Be Prevented By Holding The Temperature Of Water At 80 ºc By Steam Coil In Bottom Of Tank ? Distribution Of WFI From Storage Tank To The Point Of Use By Direct Withdrawal From The Tank Through A Pipe System.

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