ANTI-QUORUM SENSING ACTVITY OF CERTAIN MEDICINAL PLANTS

67 %
33 %
Information about ANTI-QUORUM SENSING ACTVITY OF CERTAIN MEDICINAL PLANTS
Science-Technology

Published on March 8, 2014

Author: saheedadebayo5

Source: authorstream.com

Research Progress Report on Anti-quorum sensing activity of medicinal plants on Chromobacterium violaceum: Research Progress Report on Anti-quorum sensing activity of medicinal plants on Chromobacterium violaceum BY: Ibraheem Saheed A. (3120280) Supervisor: Prof. Abdul Jalil A. Kader Progress report by Ibraheem Saheed 1 PowerPoint Presentation: CONTENTS Introduction Problem statement Objectives of the study Literature review Materials and method Results and discussion Conclusion Future work Progress report by Ibraheem Saheed 2 : Introduction Medicinal plants are natural remedies from plants, with higher safety profile and efficacy ( Trivendi et al., 2011) Multidrug resistance bacteria continue to pose more threat as antibiotics no longer effective. A promising alternative treatment is to interfere with their quorum sensing. Screening of medicinal plants for the presence of anti-quorum sensing compounds has been encouraged by the trend of the current research Progress report by Ibraheem Saheed 3 PowerPoint Presentation: Problem statement A promising treatment to the bacteria that are forming resistance against antibiotics will be to prevent them from communicating. This will prevent their virulence effect without interfering with their growth. Searching for quorum sensing inhibiting compounds has therefore become paramount, especially in natural products with good medicinal history. Progress report by Ibraheem Saheed 4 Objectives of the study:  Objectives of the study To ascertain the presence of anti-quorum sensing compounds in Parkia spaciosa . To ascertain the presence of anti-quorum sensing compounds in Centellia asiatica . To ascertain the presence of anti-quorum sensing compounds in Piper nigrum . To predict another means of reducing the virulence effect o bacteria that are resistance to antibiotics Progress report by Ibraheem Saheed 5 PowerPoint Presentation: Literature review Parkia spaciosa (stink beans) Tropical leguminous plant in the family of leguminosae . The seeds are consumed raw or as vegetable with rice Major constituents are cyclic polysulfides which are responsible for its antibacterial activity. It has hypoglycemic effect and hemgglutinatting properties. Progress report by Ibraheem Saheed 6 PowerPoint Presentation: Literature review continues Centella asiatica ( Gotu kola) Plant of tropical region belonging to the family Apiaceae . It has different names in different countries. They are used as vegetables or drink as tea juice. Constituents includes triterpines , flavonoids and volatile oils. Used in renewal of neural tissues and helps in mental calmness with good antioxidant activity. Progress report by Ibraheem Saheed 7 PowerPoint Presentation: Literature review continues Piper nigrum (Black pepper) W oody climber plant belonging to the family of piperaceae and it is a native of India but now found in most tropical regions. It has been named king of spices. Constituents includes lignans , alkaloids, flavonoids, pipering and aromatic compounds. It prevents inflammation, high blood pressure and diseases from mutation. Progress report by Ibraheem Saheed 8 PowerPoint Presentation: Literature review continues Quorum sensing This is a process by which bacteria communicate with one another using a signaling molecule called autoinducers ( Chistopher & Bassler , 2005). Mechanism of action involve production of autoinducers , binding of autoinducers to the receptor and transcriptional activation of gene expression. Many Gram negative bacteria uses acyl- homoserine lactone (AHL) as autoinducer . In Gram positive bacteria, autoinducers are small peptides that was exported out of the cell Progress report by Ibraheem Saheed 9 PowerPoint Presentation: Literature review continues Anti-quorum sensing Mechanism involved in disrupting the processes employed by bacteria to communicate without killing the bacteria (Rasmussen & Givskov , 2006). Quorum sensing inhibitors can act by preventing the production of autoinducer , by competing with autoinducers for the receptor site or by denaturing the receptor protein so that the signaling molecule produced will not be able to bind. Progress report by Ibraheem Saheed 10 PowerPoint Presentation: Literature review continues Chromobacterium violaceum C. violaceum is a Gram negative, facultative anaerobic bacillus, resides in fresh bodies of water. It produces violet metallic pigment called violecein under the influence of quorum sensing. It is therefore used as an indicator strain in quorum sensing. Quorum sensing in C. violaceum involves the use of AHL produced by LuxI protein and receptor protein LuxR on the cell memebrane . Progress report by Ibraheem Saheed 11 PowerPoint Presentation: Materials and method Progress report by Ibraheem Saheed 12 Extraction procedure PowerPoint Presentation: Materials and method Progress report by Ibraheem Saheed 13 Experimental procedure PowerPoint Presentation: Results and discussion The result of the assay as presented in the table below showed that P. spaciosa extract demonstrated varying level of violecein inhibition. Progress report by Ibraheem Saheed 14 PowerPoint Presentation: Results and discussion As shown in the figure, the well with higher concentration of the extract demonstrated higher zone of pigment inhibition. The well with LB broth showed no pigment inhibition. The bioactive compound responsible for this action is not known yet Progress report by Ibraheem Saheed 15 PowerPoint Presentation: Conclusion and recommendation The result of this study proved that P. spaciosa extract exhibited anti-quorum sensing effect on C. violaceum ATCC 12472 bioassay. However this study is not enough to establish the bioactive compound responsible for anti-quorum sensing activity of P. spaciosa . Therefore it is recommended that further research should be done to establish the bioactive compounds and their mode of action, so that therapeutic efficacy of P. spaciosa will be established. Progress report by Ibraheem Saheed 16 PowerPoint Presentation: Future work The same method will be used to investigate the anti-quorum sensing activity of Centella asiatica and Piper nigrum . The results of their methanolic extract will be compared with their ethanolic extract. Chromobacterium violaceum CV026 which can not self produce the autoinducer but respond to exogenous AHL will also be tried to understand the role of AHL in C. violaceum quorum sensing. Progress report by Ibraheem Saheed 17 PowerPoint Presentation: References Christopher M. Waters & Bonnie L. Bassler . 2005. Quorum Sensing: Cell-to-Cell Communication in Bacteria. The Annual Review of Cell and Development Biology . 21 : 319-346 Rasmussen T.B. & Givskov M. 2006. Quorum Sensing Inhibitors: a bargain of effects. Microbiology . 152 : 895-904 Trivedi M. N., Archana K., Urmila D. V., Charmi P. S. & Santani D. D. 2011. Pharmacognostic , phytochemical analysis and antimicrobial activity of two piper species. International Journal of Comprehensive Pharmacy 7 : 1-4 Progress report by Ibraheem Saheed 18 PowerPoint Presentation: Progress report by Ibraheem Saheed 19 PowerPoint Presentation: Progress report by Ibraheem Saheed 20 PowerPoint Presentation: Progress report by Ibraheem Saheed 21 PowerPoint Presentation: Progress report by Ibraheem Saheed 22 PowerPoint Presentation: Progress report by Ibraheem Saheed 23

Add a comment

Related presentations

Related pages

Plant-Derived Antimicrobial Compounds - Medscape

Could medicinal plants ... plant extracts with bacterial quorum-sensing inhibitory activity; and plant ... Ahmad I. Antibiofilm activity of certain ...
Read more

ANTIBACTERIAL ACTIVITY OF MEDICINAL PLANT EXTRACTS - SciELO

ANTIBACTERIAL ACTIVITY OF MEDICINAL PLANT EXTRACTS ... Anti-quorum sensing activity of medicinal plants in ... 15 medicinal plants against ESβL-producing ...
Read more

IJPT THERAPEUTIC PROPERTIES OF MEDICINAL PLANTS: A REVIEW ...

Previous studies showed that medicinal plants exerted a wide range of antibacterial activity. These plants Chenopodium album and Chrozophora tinctoria.
Read more

chromobacterium violaceum cv026: Topics by Science.gov

... all samples exhibited certain antioxidant activity ... Sources with Anti-Quorum Sensing ... Chinese medicinal plants for quorum-sensing ...
Read more

ar.scribd.com

ar.scribd.com
Read more

tmpC737.tmp - de.scribd.com

tmpC737.tmp - de.scribd.com
Read more