8 fem fruit breedomics mab services pindo massimo

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Information about 8 fem fruit breedomics mab services pindo massimo
Technology

Published on February 28, 2014

Author: fruitbreedomics

Source: slideshare.net

Sequencing and Genotyping Platform Applications Facilities & Services Massimo Pindo, PhD massimo.pindo@fmach.it Sequencing & Genotyping Platform Edmund Mach Foundation

Edmund Mach Foundation (since 2007) Inheritance of Ist. Agrario San Michele a/A since 1874 A non for profit organization with private legal status and over 750 staff operating in several sites in the Trentino province It provides Research, Education,Technology Transfer and Advisory Service in the Agri-Food and Environmental sectors.

FEM Research and Innovation Centre

Mission: The Sequencing and Genotyping Platform takes part in the research and development activity of the CRI by offering support at the researchers activity and with the development of new methods of experimental investigation. The first purpose of the Platform is the development of new investigative methods, with the acquisition of new knowledge in the green biotechnology field and the continuous improvement of present and future instruments. Platform facilities Platform Services Platform Equipment

Genomics and Biology of Fruit Crops Germplasm platform Apple: - 13.4 ha for apple breeding + germplasm collection - 1,200 accessions (wild and cultivated spp.) - 54,000 genotypes under evaluation /several mapping population (quality and pyramidation of resistances) - 8,000 genotypes in greenhouse Grape: - 4.5 ha for grapevine breeding + germplasm collection - 1,900 accessions (wild, hybrids and cultivated) - 12,000 new crosses (crosses, selected individuals or large mapping populations) resistances with high quality standards Softfruits: - 2.5 ha for raspberry & blueberry breeding + germplasm - 104 strawbeery accessions - 140 blueberry accessions (20,000 seedlings/48 crosses) - 240 raspberry accessions (14,300 seedlings/110 crosses)

MAS 2013 AT FEM 24 APPLE CROSSES 6.471 PLANTS - 8.068 GENOTYPING ASSAY CROSS SELECTED FOR: 12.1 12.3 12.4 12.5 12.6 12.8 12.9 12.10 12.11 12.12 12.14 12.15 12.16 12.17 12.18 12.19 12.20 12.21 12.24 12.27 12.28 12.29 12.30 12.31 disesase resistance texture texture and ethylene disesase resistance disesase resistance disesase resistance ethylene disesase resistance texture ethylene disesase resistance disesase resistance disesase resistance and texture disesase resistance texture disesase resistance disesase resistance disesase resistance and ethylene disesase resistance ethylene disesase resistance and texture disesase resistance ethylene ethylene GENE Vf Pl1 Md-PG1   Md-PG1 Md-ACO1 Vf   Vf   Vf   Md-ACO1   Vf   Md-PG1   Md-ACO1   Vh4   Vh2   Vf Md-PG1 Vf Vr2 Md-PG1   Vf SAMPLES TESTED   Vf Pl1 Vf Md-ACO1 Vf Vr2 Md-ACO1   Vf Md-PG1 Vf   Md-ACS1   Md-ACO1   69,51% Plants with desiderable traits

Marker Assisted Breeding at FEM MASTER TRAITS (ie: Resistance, Texture, Storage) - MAB consultant (management system) - gold standard markers - well optimazed flowthrough

Marker Assisted Breeding at FEM STEP 1 Seedling layout STEP 2 Sample collection STEP 3 Run Analysis

People: Sequencing and Genotyping Platform Erika Stefani Daniela Nicolini Next Gen. Sequencing Next Gen. Sequencing Next Gen. Sequencing and Next Gen. Sequencing and Protocol Setting Protocol Setting Whole Genome Seq. Whole Genome Seq. Target Reseq. Target Reseq. Metagenomics Metagenomics Transcriptome Seq. Transcriptome Seq. Shot gun & PE gDNA library Shot gun & PE gDNA library Amplicon Library Amplicon Library Next Gen. Sequencing Next Gen. Sequencing Application Application Massimo Pindo Simone Larger Elisa Banchi Genotyping & Genotyping & Robotic Platform Robotic Platform DNA & RNA extraction DNA & RNA extraction Capillary Sequencing Capillary Sequencing Mid- to High-plex Genotyping Mid- to High-plex Genotyping Lara Poles

People: Sequencing and Genotyping Platform Erika Stefani Daniela Nicolini Next Gen. Sequencing Next Gen. Sequencing Next Gen. Sequencing and Next Gen. Sequencing and Protocol Setting Protocol Setting Whole Genome Seq. Whole Genome Seq. Target Reseq. Target Reseq. Metagenomics Metagenomics Transcriptome Seq. Transcriptome Seq. Shot gun & PE gDNA library Shot gun & PE gDNA library Amplicon Library Amplicon Library Next Gen. Sequencing Next Gen. Sequencing Application Application Massimo Pindo Simone Larger Elisa Banchi Genotyping & Genotyping & Robotic Platform Robotic Platform DNA & RNA extraction DNA & RNA extraction Capillary Sequencing Capillary Sequencing Mid- to High-plex Genotyping Mid- to High-plex Genotyping Lara Poles

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