081205 Journal Club

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Published on December 5, 2008

Author: AndrewHires

Source: slideshare.net

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Journal Club of Rodent Somatosensory Cortex SII

Journal Club Mouse Somatosensory Cortex SII Andrew Hires 12.5.2008

Mouse Somatosensory Cortex SII Where is it? What is its structure? What is its response characteristics? What is its function?

Where is it?

What is its structure?

What is its response characteristics?

What is its function?

Location of SII 4mm 2 "Gran Torino" 4mm 2

4mm 2

Carvell & Simons 1986 Sens & Motor Res Sodium pentobarbital Tungsten microelectrodes Normal to pia, 300-450um (III, IV) most vigor Poking, brushing, tapping glass

Sodium pentobarbital

Tungsten microelectrodes

Normal to pia, 300-450um (III, IV) most vigor

Poking, brushing, tapping glass

Somatosensory response map in SII Whole body somatotopic map Larger receptive fields “ head up” orientation map

Only contralateral input in whisker area SI to SII transition and whisker area

Only contralateral input in whisker area

Subdivisions of SII Carvell & Simons 1986 Whiskers are most medial Face is 54% of SII, Whiskers are 16% of SII Auditory overlap in lateral and caudal areas (not in whisker)

SII cytoarchitecture No barrels Thickening of cortex Diffuse IV, less uniform lamination of V

No barrels

Thickening of cortex

Diffuse IV, less uniform lamination of V

SII inputs Thalamic Input POm (latency coding), VPMvl Paralemniscal pathway Parallel stream Cortical Input Major from SI Also surrounding cortical areas

Thalamic Input

POm (latency coding), VPMvl

Paralemniscal pathway

Parallel stream

Cortical Input

Major from SI

Also surrounding cortical areas

Chakrabarti & Alloway 2006- SII Injections Retrograde tracers into SII (and MI) Diamidino yellow, true blue CTb, BDA, Fluoro Gold 50 degree from midline 1200-1400, 1000, 800um below pia

Retrograde tracers into SII (and MI)

Diamidino yellow, true blue

CTb, BDA, Fluoro Gold

50 degree from midline

1200-1400, 1000, 800um below pia

Promiscuous SII Inputs Big injection SI Input from barrel and septa

Big injection

SI Input from barrel and septa

MI has septa preference, SII does not

Broadly somatotopic input to SII Same pattern in various layers

Same pattern in various layers

SII has few inputs from layer IV SI

SII and MI injections MI prefers border of septal and barrel

MI prefers border of septal and barrel

Do individual SI cells project to both?

Yes.

Thalamic input of SII and MI somewhat intermingled

VPMvl projections Pierret et al. 20 (19): 7455. (2000)

Pierret et al. 20 (19): 7455. (2000)

Kwegyir-Afful & Keller - Rat SII physiology Halothane -> fentanyl, or urethane Extracellular platinum, quartz ins, trodes Manual whisker stim 8 dir piezo stim, 50x angle 1cm from base 200ms ramp and hold 1Hz Kwegyir-Afful & Keller - 2004

Halothane -> fentanyl, or urethane

Extracellular platinum, quartz ins, trodes

Manual whisker stim

8 dir piezo stim, 50x angle

1cm from base

200ms ramp and hold

1Hz

SII vs. SI spiking properties SII latency = across layers, SI-IV ~9ms Rate < SI

SII latency = across layers, SI-IV

~9ms

Rate < SI

Stimulus dependent firing in SII 90% 10%

Large receptive fields in SII 5-12 whiskers per recording site Layer V neuron

5-12 whiskers per recording site

“ Primary” vs. “Accessory” whiskers? Top 3 ave whisker amp sig dif from rest

Top 3 ave whisker amp sig dif from rest

SII angular tuning examples Cat 6 selective Cat 0 selective

Cat 6 selective

SII more angularly selective than SI VI more selective than other layers But not significant

VI more selective than other layers

But not significant

Summary of SII response properties

Additional SII findings Evoked activity in SII is lower Preference for multiple whisker stimulation? Preference for active whisking? (Paralemniscial) RSU = FSU magnitude Less feed forward inhibition Nearly all are rapidly adapting neurons Inactivation of SI in cat does not block SII Does block in primate (hierarchal or subdivisions) Driving input is from thalamus? SI input modulates?

Evoked activity in SII is lower

Preference for multiple whisker stimulation?

Preference for active whisking? (Paralemniscial)

RSU = FSU magnitude

Less feed forward inhibition

Nearly all are rapidly adapting neurons

Inactivation of SI in cat does not block SII

Does block in primate (hierarchal or subdivisions)

Driving input is from thalamus? SI input modulates?

What is SII’s function? Receives lemniscal and paralemniscal input Whisking frequency and external stimulus Context integration in stimulus detection? Spatial distributed feature analysis? Size of objects (# of whiskers struck)

Receives lemniscal and paralemniscal input

Whisking frequency and external stimulus

Context integration in stimulus detection?

Spatial distributed feature analysis?

Size of objects (# of whiskers struck)

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