02 Asc H Continued Chivkula

50 %
50 %
Information about 02 Asc H Continued Chivkula
Health & Medicine

Published on November 13, 2008

Author: vshidham

Source: slideshare.net

Description

A potpourri of ASC-H and related interpretations-
(Part II)

Mamatha Chivukula MD, FASCP, FCAP Assistant Professor Associate Director of Immunohistochemistry lab Magee-Women’s Hospital of UPMC Pittsburgh, PA Study cases and application of two-color immunochemistry

Objectives Present real time case studies to demonstrate the utility of cytomorphology in interpretation of ASC-H pap tests Cytohisto correlation and follow-up HPV testing

Present real time case studies to demonstrate the utility of cytomorphology in interpretation of ASC-H pap tests

Cytohisto correlation and follow-up HPV testing

Cytomorphology of ASC-H MGH-like Repair like Atrophy-like- with single cells Atrophy-like- with HCG of parabasal cell groups ASC-H: NOS Cyanophilic small atypical parakeratotic cells HSIL- syncytial HSIL- Single cell

MGH-like

Repair like

Atrophy-like- with single cells

Atrophy-like- with HCG of parabasal cell groups

ASC-H: NOS

Cyanophilic small atypical parakeratotic cells

HSIL- syncytial

HSIL- Single cell

Case 1 35 year old woman w/ a routine Pap test

35 year old woman w/ a routine Pap test

 

 

Groups of atypical cells (without distinct cell borders)

-

Hyperchromatic nuclei - Coarse to fine chromatin

Follow up HPV –DNA test is Positive for high risk HPV types

Follow up HPV –DNA test is Positive for high risk HPV types

 

“ Syncytial cell” pattern Source: Chivukula M , Shidham V . CytoJournal 2006, 3:14

Case 2 49 year old woman w/ a routine Pap test

49 year old woman w/ a routine Pap test

- Abundant blue cytoplasm Open chromatin , w/w out nucleoli

- Abundant blue cytoplasm

Open chromatin , w/w out nucleoli

Follow up HPV –DNA test is Negative for high risk HPV types

Follow up HPV –DNA test is Negative for high risk HPV types

 

p16

KI -67

Atrophic “ Single-cell” pattern Source: Chivukula M , Shidham V . CytoJournal 2006, 3:14

Case 3 30 year old woman w/ a routine Pap test

30 year old woman w/ a routine Pap test

 

Cohesive clusters

-Prominent nucleoli - Low N/C ratio

Follow up HPV –DNA test is Negative for high risk HPV types

Follow up HPV –DNA test is Negative for high risk HPV types

 

“ School of fish” Repair – like pattern Source: Chivukula M , Shidham V . CytoJournal 2006, 3:14

Case 4 35 year old woman w/ a routine Pap test

35 year old woman w/ a routine Pap test

 

 

 

Follow up HPV –DNA test is Positive for high risk HPV types

Follow up HPV –DNA test is Positive for high risk HPV types

 

“ Isolated cell pattern” Source: Chivukula M , Shidham V . CytoJournal 2006, 3:14

Case 5 55 year old woman w/ a routine Pap test

55 year old woman w/ a routine Pap test

 

- HCG of parabasal cells - Small dark nuclei - Variable, usually scant cytoplasm

Follow up HPV –DNA test is Negative for high risk HPV types

Follow up HPV –DNA test is Negative for high risk HPV types

 

Atrophic “ Parabasal cell groups” Source: Chivukula M , Shidham V . CytoJournal 2006, 3:14

Application of two-color immunochemistry P16 is over expressed and accumulates in nucleus and cytoplasm of dysplastic cervical cells Studies on ASC-US, ASC-H Advanced kits for immunostaining cervical specimens to detect both p16 and ki-67 simulatneously

P16 is over expressed and accumulates in nucleus and cytoplasm of dysplastic cervical cells

Studies on ASC-US, ASC-H

Advanced kits for immunostaining cervical specimens to detect both p16 and ki-67 simulatneously

In a normal cell w/ intact cell cycle, Simultaneous expression of anti-proliferative marker (p16), proliferative marker (ki-67) within the same cell, mutually excludes each other Reversely, presence of both p16, ki-67 in the same cell, indicates disrupted cell cycle State of the art….work in progress

In a normal cell w/ intact cell cycle, Simultaneous expression of anti-proliferative marker (p16), proliferative marker (ki-67) within the same cell, mutually excludes each other

Reversely, presence of both p16, ki-67 in the same cell, indicates disrupted cell cycle

 

 

Thank you Thank you

Thank you

Add a comment

Related presentations

Related pages

Katalogbild 80/02 - BMW 315 - 323i (E21) - Domstreben ...

Katalogbild 80/02 Ersatzteile. Motorteile / Kühlung; Zündung / Motorelektrik; Vergaser / Einspritzung; Tank; Auspuffanlage; Kupplung u. Betätigung ...
Read more

AWARD APPLICATION - EHS Today

... EHS Today’s America’s Safest Companies Award Application Safety Performance (continued) 12. ... (total from Column H on your OSHA Form)
Read more

ASCII-Tabelle - Torsten Horn, Ingenieurbüro für ...

^h ^i ^j ^k ^l ^m ^n ^o ^p ^q ^r ^s ^t ^u ... 02 03 29 05 06 07 0d 09 0a 1b 1b 33 35 34 08 0b 02 03 04 05 06 07 08 09 0a 34 33 2b 0b 2b 0c: 20 21 22 23 24 ...
Read more

Full-text - Should LSIL with ASC-H (LSIL-H) be an ...

Official Full-Text Publication: Should LSIL with ASC-H ... Another alternative to LSIL-H as a distinct category is to continue with ... Chivkula M , Cafaro ...
Read more

Follow-up of low-grade abnormal Pap tests

Topics will continue to be in English. ... Follow-up of low-grade abnormal Pap tests ... (ASC-H) — This finding ...
Read more

www.igcs.org

... (ASC - H) Colposcopic Examination NO CIN Cytology@ 6 & 12 mos OR ... continue observation Other Results ... 02:30 AM ...
Read more

40 CFR Ch. I (7-1-02 Edition) Environmental Protection ...

... (Continued) 3 Finding Aids: ... The Code of Federal Regulations is a codification of the general and permanent rules published in the Federal Register ...
Read more

GEWINNZAHLEN - KENO von LOTTO

Dieser Statistik liegen 4154 Ziehungen vom 02.02.2004 bis 13.05.2016 zugrunde.
Read more